Single-frame 3D fluorescence microscopy with ultraminiature lensless FlatScope

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dc.citation.articleNumbere1701548
dc.citation.issueNumber12
dc.citation.journalTitleScience Advances
dc.citation.volumeNumber3
dc.contributor.authorAdams, Jesse K.
dc.contributor.authorBoominathan, Vivek
dc.contributor.authorAvants, Benjamin W.
dc.contributor.authorVercosa, Daniel G.
dc.contributor.authorYe, Fan
dc.contributor.authorBaraniuk, Richard G.
dc.contributor.authorRobinson, Jacob T.
dc.contributor.authorVeeraraghavan, Ashok
dc.contributor.orgNanophotonic Computational Imaging and Sensing Laboratory
dc.date.accessioned2018-06-27T12:34:33Z
dc.date.available2018-06-27T12:34:33Z
dc.date.issued2017
dc.description.abstractModern biology increasingly relies on fluorescence microscopy, which is driving demand for smaller, lighter, and cheaper microscopes. However, traditional microscope architectures suffer from a fundamental trade-off: As lenses become smaller, they must either collect less light or image a smaller field of view. To break this fundamental trade-off between device size and performance, we present a new concept for three-dimensional (3D) fluorescence imaging that replaces lenses with an optimized amplitude mask placed a few hundred micrometers above the sensor and an efficient algorithm that can convert a single frame of captured sensor data into high-resolution 3D images. The result is FlatScope: perhaps the world's tiniest and lightest microscope. FlatScope is a lensless microscope that is scarcely larger than an image sensor (roughly 0.2 g in weight and less than 1 mm thick) and yet able to produce micrometer-resolution, high-frame rate, 3D fluorescence movies covering a total volume of several cubic millimeters. The ability of FlatScope to reconstruct full 3D images from a single frame of captured sensor data allows us to image 3D volumes roughly 40,000 times faster than a laser scanning confocal microscope while providing comparable resolution. We envision that this new flat fluorescence microscopy paradigm will lead to implantable endoscopes that minimize tissue damage, arrays of imagers that cover large areas, and bendable, flexible microscopes that conform to complex topographies.
dc.identifier.citationAdams, Jesse K., Boominathan, Vivek, Avants, Benjamin W., et al.. "Single-frame 3D fluorescence microscopy with ultraminiature lensless FlatScope." <i>Science Advances,</i> 3, no. 12 (2017) AAAS: https://doi.org/10.1126/sciadv.1701548.
dc.identifier.digitale1701548.full
dc.identifier.doihttps://doi.org/10.1126/sciadv.1701548
dc.identifier.urihttps://hdl.handle.net/1911/102284
dc.language.isoeng
dc.publisherAAAS
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.titleSingle-frame 3D fluorescence microscopy with ultraminiature lensless FlatScope
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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