Tracking neural crest cell cycle progressionﾠin vivo

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dc.citation.articleNumbere23214en_US
dc.citation.issueNumber7-Junen_US
dc.citation.journalTitlegenesisen_US
dc.citation.volumeNumber56en_US
dc.contributor.authorRajan, Sriivatsan G.en_US
dc.contributor.authorGallik, Kristin L.en_US
dc.contributor.authorMonaghan, James R.en_US
dc.contributor.authorUribe, Rosa Annaen_US
dc.contributor.authorBronner, Marianne E.en_US
dc.contributor.authorSaxena, Ankuren_US
dc.date.accessioned2019-08-28T16:10:12Zen_US
dc.date.available2019-08-28T16:10:12Zen_US
dc.date.issued2018en_US
dc.description.abstractAnalysis of cell cycle entry/exit and progression can provide fundamental insights into stem cell propagation, maintenance, and differentiation. The neural crest is a unique stem cell population in vertebrate embryos that undergoes long-distance collective migration and differentiation into a wide variety of derivatives. Using traditional techniques such as immunohistochemistry to track cell cycle changes in such a dynamic population is challenging, as static time points provide an incomplete spatiotemporal picture. In contrast, the fluorescent, ubiquitination-based cell cycle indicator (Fucci) system provides in vivo readouts of cell cycle progression and has been previously adapted for use in zebrafish. The most commonly used Fucci systems are ubiquitously expressed, making tracking of a specific cell population challenging. Therefore, we generated a transgenic zebrafish line, Tg(-4.9sox10:mAG-gmnn(1/100)-2A-mCherry-cdt1(1/190)), in which the Fucci system is specifically expressed in delaminating and migrating neural crest cells. Here, we demonstrate validation of this new tool and its use in live high-resolution tracking of cell cycle progression in the neural crest and derivative populations.en_US
dc.identifier.citationRajan, Sriivatsan G., Gallik, Kristin L., Monaghan, James R., et al.. "Tracking neural crest cell cycle progressionﾠin vivo." <i>genesis,</i> 56, no. 6-7 (2018) Wiley: https://doi.org/10.1002/dvg.23214.en_US
dc.identifier.doihttps://doi.org/10.1002/dvg.23214en_US
dc.identifier.urihttps://hdl.handle.net/1911/107370en_US
dc.language.isoengen_US
dc.publisherWileyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.en_US
dc.subject.keywordFuccien_US
dc.subject.keywordSox10en_US
dc.subject.keywordzebrafishen_US
dc.titleTracking neural crest cell cycle progressionﾠin vivoen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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