Xyloglucan Endotransglucosylase-Hydrolase17 Interacts with Xyloglucan Endotransglucosylase-Hydrolase31 to Confer Xyloglucan Endotransglucosylase Action and Affect Aluminum Sensitivity in Arabidopsis

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dc.citation.firstpage1566en_US
dc.citation.issueNumber4en_US
dc.citation.journalTitlePlant Physiologyen_US
dc.citation.lastpage1574en_US
dc.citation.volumeNumber165en_US
dc.contributor.authorZhu, Xiao Fangen_US
dc.contributor.authorWan, Jiang Xueen_US
dc.contributor.authorSun, Yingen_US
dc.contributor.authorShi, Yuan Zhien_US
dc.contributor.authorBraam, Janeten_US
dc.contributor.authorLi, Gui Xinen_US
dc.contributor.authorZheng, Shao Jianen_US
dc.date.accessioned2015-07-09T20:14:49Zen_US
dc.date.available2015-07-09T20:14:49Zen_US
dc.date.issued2014en_US
dc.description.abstractPreviously, we reported that although the Arabidopsis (Arabidopsis thaliana) Xyloglucan Endotransglucosylase-Hydrolase31 (XTH31) has predominately xyloglucan endohydrolase activity in vitro, loss of XTH31 results in remarkably reduced in vivo xyloglucan endotransglucosylase (XET) action and enhanced Al resistance. Here, we report that XTH17, predicted to have XET activity, binds XTH31 in yeast (Saccharomyces cerevisiae) two-hybrid and coimmunoprecipitations assays and that this interaction may be required for XTH17 XET activity in planta. XTH17 and XTH31 may be colocalized in plant cells because tagged XTH17 fusion proteins, like XTH31 fusion proteins, appear to target to the plasma membrane. XTH17 expression, like that of XTH31, was substantially reduced in the presence of aluminum (Al), even at concentrations as low as 10 ﾵm for 24 h or 25 ﾵm for just 30 min. Agrobacterium tumefaciens-mediated transfer DNA insertion mutant of XTH17, xth17, showed low XET action and had moderately shorter roots than the wild type but was more Al resistant than the wild type. Similar to xth31, xth17 had low hemicellulose content and retained less Al in the cell wall. These data suggest a model whereby XTH17 and XTH31 may exist as a dimer at the plasma membrane to confer in vivo XET action, which modulates cell wall Al-binding capacity and thereby affects Al sensitivity in Arabidopsis.en_US
dc.identifier.citationZhu, Xiao Fang, Wan, Jiang Xue, Sun, Ying, et al.. "Xyloglucan Endotransglucosylase-Hydrolase17 Interacts with Xyloglucan Endotransglucosylase-Hydrolase31 to Confer Xyloglucan Endotransglucosylase Action and Affect Aluminum Sensitivity in Arabidopsis." <i>Plant Physiology,</i> 165, no. 4 (2014) American Society of Plant Biologists: 1566-1574. http://dx.doi.org/10.1104/pp.114.243790.en_US
dc.identifier.doihttp://dx.doi.org/10.1104/pp.114.243790en_US
dc.identifier.urihttps://hdl.handle.net/1911/80862en_US
dc.language.isoengen_US
dc.publisherAmerican Society of Plant Biologistsen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.titleXyloglucan Endotransglucosylase-Hydrolase17 Interacts with Xyloglucan Endotransglucosylase-Hydrolase31 to Confer Xyloglucan Endotransglucosylase Action and Affect Aluminum Sensitivity in Arabidopsisen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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