A photoconversion model for full spectral programming and multiplexing of optogeneticﾠsystems

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dc.citation.articleNumber926en_US
dc.citation.journalTitleMolecular Systems Biologyen_US
dc.citation.volumeNumber13en_US
dc.contributor.authorOlson, Evan J.en_US
dc.contributor.authorTzouanas, Constantine N.en_US
dc.contributor.authorTabor, Jeffrey J.en_US
dc.contributor.orgBioengineeringen_US
dc.contributor.orgBiosciencesen_US
dc.date.accessioned2017-05-04T18:16:35Zen_US
dc.date.available2017-05-04T18:16:35Zen_US
dc.date.issued2017en_US
dc.description.abstractOptogenetics combines externally applied light signals and genetically engineered photoreceptors to control cellular processes with unmatched precision. Here, we develop a mathematical model of wavelength‐ and intensity‐dependent photoconversion, signaling, and output gene expression for our two previously engineered light‐sensing Escherichia coli two‐component systems. To parameterize the model, we develop a simple set of spectral and dynamical calibration experiments using our recent open‐source “Light Plate Apparatus” device. In principle, the parameterized model should predict the gene expression response to any time‐varying signal from any mixture of light sources with known spectra. We validate this capability experimentally using a suite of challenging light sources and signals very different from those used during the parameterization process. Furthermore, we use the model to compensate for significant spectral cross‐reactivity inherent to the two sensors in order to develop a new method for programming two simultaneous and independent gene expression signals within the same cell. Our optogenetic multiplexing method will enable powerful new interrogations of how metabolic, signaling, and decision‐making pathways integrate multiple input signals.en_US
dc.identifier.citationOlson, Evan J., Tzouanas, Constantine N. and Tabor, Jeffrey J.. "A photoconversion model for full spectral programming and multiplexing of optogeneticﾠsystems." <i>Molecular Systems Biology,</i> 13, (2017) EMBO Press: https://doi.org/10.15252/msb.20167456.en_US
dc.identifier.doihttps://doi.org/10.15252/msb.20167456en_US
dc.identifier.urihttps://hdl.handle.net/1911/94172en_US
dc.language.isoengen_US
dc.publisherEMBO Pressen_US
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordoptogeneticsen_US
dc.subject.keywordpredictive modelingen_US
dc.subject.keywordsynthetic biologyen_US
dc.subject.keywordspectral multiplexingen_US
dc.subject.keywordtwo-component systemsen_US
dc.titleA photoconversion model for full spectral programming and multiplexing of optogeneticﾠsystemsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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