Superhydrophobic Array Devices for the Enhanced Formation of 3D Cancer Models

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dc.citation.firstpage23637en_US
dc.citation.issueNumber34en_US
dc.citation.journalTitleACS Nanoen_US
dc.citation.lastpage23654en_US
dc.citation.volumeNumber18en_US
dc.contributor.authorLopez-Cavestany, Mariaen_US
dc.contributor.authorWright, Olivia A.en_US
dc.contributor.authorReckhorn, Noah T.en_US
dc.contributor.authorCarter, Alexandria T.en_US
dc.contributor.authorJayawardana, Kalanaen_US
dc.contributor.authorNguyen, Tinen_US
dc.contributor.authorBriggs, Dayrl P.en_US
dc.contributor.authorKoktysh, Dmitry S.en_US
dc.contributor.authorEsteban Linares, Albertoen_US
dc.contributor.authorLi, Deyuen_US
dc.contributor.authorKing, Michael R.en_US
dc.date.accessioned2024-10-08T13:27:49Zen_US
dc.date.available2024-10-08T13:27:49Zen_US
dc.date.issued2024en_US
dc.description.abstractDuring the metastatic cascade, cancer cells travel through the bloodstream as circulating tumor cells (CTCs) to a secondary site. Clustered CTCs have greater shear stress and treatment resistance, yet their biology remains poorly understood. We therefore engineered a tunable superhydrophobic array device (SHArD). The SHArD-C was applied to culture a clinically relevant model of CTC clusters. Using our device, we cultured a model of cancer cell aggregates of various sizes with immortalized cancer cell lines. These exhibited higher E-cadherin expression and are significantly more capable of surviving high fluid shear stress-related forces compared to single cells and model clusters grown using the control method, helping to explain why clustering may provide a metastatic advantage. Additionally, the SHArD-S, when compared with the AggreWell 800 method, provides a more consistent spheroid-forming device culturing reproducible sizes of spheroids for multiple cancer cell lines. Overall, we designed, fabricated, and validated an easily tunable engineered device which grows physiologically relevant three-dimensional (3D) cancer models containing tens to thousands of cells.en_US
dc.identifier.citationLopez-Cavestany, M., Wright, O. A., Reckhorn, N. T., Carter, A. T., Jayawardana, K., Nguyen, T., Briggs, D. P., Koktysh, D. S., Esteban Linares, A., Li, D., & King, M. R. (2024). Superhydrophobic Array Devices for the Enhanced Formation of 3D Cancer Models. ACS Nano, 18(34), 23637–23654. https://doi.org/10.1021/acsnano.4c08132en_US
dc.identifier.digitalsuperhydrophobic-array-devicesen_US
dc.identifier.doihttps://doi.org/10.1021/acsnano.4c08132en_US
dc.identifier.urihttps://hdl.handle.net/1911/117929en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsExcept where otherwise noted, this work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND) license. Permission to reuse, publish, or reproduce the work beyond the terms of the license or beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.titleSuperhydrophobic Array Devices for the Enhanced Formation of 3D Cancer Modelsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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