Optimization of PAMAM-gold nanoparticle conjugation for gene therapy

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dc.citation.firstpage1725
dc.citation.issueNumber5
dc.citation.journalTitleBiomaterials
dc.citation.lastpage1734
dc.citation.volumeNumber35
dc.contributor.authorFigueroa, Elizabeth R.
dc.contributor.authorLin, Adam Y.
dc.contributor.authorYan, Jiaxi
dc.contributor.authorLuo, Laureen
dc.contributor.authorFoster, Aaron E.
dc.contributor.authorDrezek, Rebekah A.
dc.date.accessioned2015-03-16T16:56:09Z
dc.date.available2015-03-16T16:56:09Z
dc.date.issued2014
dc.description.abstractThe development of efficient and biocompatible non-viral vectors for gene therapy remains a great challenge, and exploiting the properties of both nanoparticle carriers and cationic polymers is an attractive approach. In this work, we have developed gold nanoparticle (AuNP) polyamidoamine (PAMAM) conjugates for use as non-viral transfection agents. AuPAMAM conjugates were prepared by crosslinking PAMAM dendrimers to carboxylic-terminated AuNPs via EDC and sulfo-NHS chemistry. EDC and sulfo-NHS have been utilized widely and in numerous applications such as amino acid coupling; however, their use in the coupling of PAMAM dendrimers to AuNPs presents new challenges to form effective and stable constructs for delivery that have not yet been examined. Enhanced colloidal stability and DNA condensation ability was established by probing two critical synthetic parameters: the reaction rate of the PAMAM crosslinking step, and the amine to carboxyl ratio. Based on this work, increasing the amine to carboxyl ratio during conjugation of PAMAM onto AuNPs yielded the optimal vector with respect to colloidal stability and transfection efficiency inﾠvitro. AuPAMAM conjugates present attractive candidates for non-viral gene delivery due to their commercial availability, ease of fabrication and scale-up, high yield, high transfection efficiency and low cytotoxicity.
dc.identifier.citationFigueroa, Elizabeth R., Lin, Adam Y., Yan, Jiaxi, et al.. "Optimization of PAMAM-gold nanoparticle conjugation for gene therapy." <i>Biomaterials,</i> 35, no. 5 (2014) Elsevier: 1725-1734. http://dx.doi.org/10.1016/j.biomaterials.2013.11.026.
dc.identifier.doihttp://dx.doi.org/10.1016/j.biomaterials.2013.11.026
dc.identifier.urihttps://hdl.handle.net/1911/79346
dc.language.isoeng
dc.publisherElsevier
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.subject.keywordgold
dc.subject.keywordnanoparticle
dc.subject.keywordnanocomposite
dc.subject.keywordgene therapy
dc.subject.keyworddendrimer
dc.subject.keywordDNA
dc.titleOptimization of PAMAM-gold nanoparticle conjugation for gene therapy
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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