Optimization of PAMAM-gold nanoparticle conjugation for gene therapy

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dc.citation.firstpage1725en_US
dc.citation.issueNumber5en_US
dc.citation.journalTitleBiomaterialsen_US
dc.citation.lastpage1734en_US
dc.citation.volumeNumber35en_US
dc.contributor.authorFigueroa, Elizabeth R.en_US
dc.contributor.authorLin, Adam Y.en_US
dc.contributor.authorYan, Jiaxien_US
dc.contributor.authorLuo, Laureenen_US
dc.contributor.authorFoster, Aaron E.en_US
dc.contributor.authorDrezek, Rebekah A.en_US
dc.date.accessioned2015-03-16T16:56:09Zen_US
dc.date.available2015-03-16T16:56:09Zen_US
dc.date.issued2014en_US
dc.description.abstractThe development of efficient and biocompatible non-viral vectors for gene therapy remains a great challenge, and exploiting the properties of both nanoparticle carriers and cationic polymers is an attractive approach. In this work, we have developed gold nanoparticle (AuNP) polyamidoamine (PAMAM) conjugates for use as non-viral transfection agents. AuPAMAM conjugates were prepared by crosslinking PAMAM dendrimers to carboxylic-terminated AuNPs via EDC and sulfo-NHS chemistry. EDC and sulfo-NHS have been utilized widely and in numerous applications such as amino acid coupling; however, their use in the coupling of PAMAM dendrimers to AuNPs presents new challenges to form effective and stable constructs for delivery that have not yet been examined. Enhanced colloidal stability and DNA condensation ability was established by probing two critical synthetic parameters: the reaction rate of the PAMAM crosslinking step, and the amine to carboxyl ratio. Based on this work, increasing the amine to carboxyl ratio during conjugation of PAMAM onto AuNPs yielded the optimal vector with respect to colloidal stability and transfection efficiency inﾠvitro. AuPAMAM conjugates present attractive candidates for non-viral gene delivery due to their commercial availability, ease of fabrication and scale-up, high yield, high transfection efficiency and low cytotoxicity.en_US
dc.identifier.citationFigueroa, Elizabeth R., Lin, Adam Y., Yan, Jiaxi, et al.. "Optimization of PAMAM-gold nanoparticle conjugation for gene therapy." <i>Biomaterials,</i> 35, no. 5 (2014) Elsevier: 1725-1734. http://dx.doi.org/10.1016/j.biomaterials.2013.11.026.en_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.biomaterials.2013.11.026en_US
dc.identifier.urihttps://hdl.handle.net/1911/79346en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.en_US
dc.subject.keywordgolden_US
dc.subject.keywordnanoparticleen_US
dc.subject.keywordnanocompositeen_US
dc.subject.keywordgene therapyen_US
dc.subject.keyworddendrimeren_US
dc.subject.keywordDNAen_US
dc.titleOptimization of PAMAM-gold nanoparticle conjugation for gene therapyen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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