Extracellular electron transfer increases fermentation in lactic acid bacteria via a hybrid metabolism

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dc.citation.articleNumbere70684
dc.citation.journalTitleeLife
dc.citation.volumeNumber11
dc.contributor.authorTejedor-Sanz, Sara
dc.contributor.authorStevens, Eric T
dc.contributor.authorLi, Siliang
dc.contributor.authorFinnegan, Peter
dc.contributor.authorNelson, James
dc.contributor.authorKnoesen, Andre
dc.contributor.authorLight, Samuel H
dc.contributor.authorAjo-Franklin, Caroline M
dc.contributor.authorMarco, Maria L
dc.date.accessioned2022-03-24T13:31:38Z
dc.date.available2022-03-24T13:31:38Z
dc.date.issued2022
dc.description.abstractEnergy conservation in microorganisms is classically categorized into respiration and fermentation; however, recent work shows some species can use mixed or alternative bioenergetic strategies. We explored the use of extracellular electron transfer for energy conservation in diverse lactic acid bacteria (LAB), microorganisms that mainly rely on fermentative metabolism and are important in food fermentations. The LAB Lactiplantibacillus plantarum uses extracellular electron transfer to increase its NAD+/NADH ratio, generate more ATP through substrate-level phosphorylation, and accumulate biomass more rapidly. This novel, hybrid metabolism is dependent on a type-II NADH dehydrogenase (Ndh2) and conditionally requires a flavin-binding extracellular lipoprotein (PplA) under laboratory conditions. It confers increased fermentation product yield, metabolic flux, and environmental acidification in laboratory media and during kale juice fermentation. The discovery of a single pathway that simultaneously blends features of fermentation and respiration in a primarily fermentative microorganism expands our knowledge of energy conservation and provides immediate biotechnology applications.
dc.identifier.citationTejedor-Sanz, Sara, Stevens, Eric T, Li, Siliang, et al.. "Extracellular electron transfer increases fermentation in lactic acid bacteria via a hybrid metabolism." <i>eLife,</i> 11, (2022) eLife Sciences Publications Ltd.: https://doi.org/10.7554/eLife.70684.
dc.identifier.digitalelife-70684-v2
dc.identifier.doihttps://doi.org/10.7554/eLife.70684
dc.identifier.urihttps://hdl.handle.net/1911/112039
dc.language.isoeng
dc.publishereLife Sciences Publications Ltd.
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleExtracellular electron transfer increases fermentation in lactic acid bacteria via a hybrid metabolism
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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