Extracellular electron transfer increases fermentation in lactic acid bacteria via a hybrid metabolism

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dc.citation.articleNumbere70684en_US
dc.citation.journalTitleeLifeen_US
dc.citation.volumeNumber11en_US
dc.contributor.authorTejedor-Sanz, Saraen_US
dc.contributor.authorStevens, Eric Ten_US
dc.contributor.authorLi, Siliangen_US
dc.contributor.authorFinnegan, Peteren_US
dc.contributor.authorNelson, Jamesen_US
dc.contributor.authorKnoesen, Andreen_US
dc.contributor.authorLight, Samuel Hen_US
dc.contributor.authorAjo-Franklin, Caroline Men_US
dc.contributor.authorMarco, Maria Len_US
dc.date.accessioned2022-03-24T13:31:38Zen_US
dc.date.available2022-03-24T13:31:38Zen_US
dc.date.issued2022en_US
dc.description.abstractEnergy conservation in microorganisms is classically categorized into respiration and fermentation; however, recent work shows some species can use mixed or alternative bioenergetic strategies. We explored the use of extracellular electron transfer for energy conservation in diverse lactic acid bacteria (LAB), microorganisms that mainly rely on fermentative metabolism and are important in food fermentations. The LAB Lactiplantibacillus plantarum uses extracellular electron transfer to increase its NAD+/NADH ratio, generate more ATP through substrate-level phosphorylation, and accumulate biomass more rapidly. This novel, hybrid metabolism is dependent on a type-II NADH dehydrogenase (Ndh2) and conditionally requires a flavin-binding extracellular lipoprotein (PplA) under laboratory conditions. It confers increased fermentation product yield, metabolic flux, and environmental acidification in laboratory media and during kale juice fermentation. The discovery of a single pathway that simultaneously blends features of fermentation and respiration in a primarily fermentative microorganism expands our knowledge of energy conservation and provides immediate biotechnology applications.en_US
dc.identifier.citationTejedor-Sanz, Sara, Stevens, Eric T, Li, Siliang, et al.. "Extracellular electron transfer increases fermentation in lactic acid bacteria via a hybrid metabolism." <i>eLife,</i> 11, (2022) eLife Sciences Publications Ltd.: https://doi.org/10.7554/eLife.70684.en_US
dc.identifier.digitalelife-70684-v2en_US
dc.identifier.doihttps://doi.org/10.7554/eLife.70684en_US
dc.identifier.urihttps://hdl.handle.net/1911/112039en_US
dc.language.isoengen_US
dc.publishereLife Sciences Publications Ltd.en_US
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are crediteden_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleExtracellular electron transfer increases fermentation in lactic acid bacteria via a hybrid metabolismen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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