Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers

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dc.citation.firstpage443en_US
dc.citation.journalTitleNature Methodsen_US
dc.citation.lastpage449en_US
dc.citation.volumeNumber14en_US
dc.contributor.authorFuller, Franklin D.en_US
dc.contributor.authorGul, Sherazen_US
dc.contributor.authorChatterjee, Ruchiraen_US
dc.contributor.authorBurgie, E.Setheen_US
dc.contributor.authorYoung, Iris D.en_US
dc.contributor.authorLebrette, Hugoen_US
dc.contributor.authorSrinivas, Viveken_US
dc.contributor.authorBrewster, Aaron S.en_US
dc.contributor.authorMichels-Clark, Taraen_US
dc.contributor.authorClinger, Jonathan A.en_US
dc.contributor.authorAndi, Babaken_US
dc.contributor.authorIbrahim, Mohameden_US
dc.contributor.authorPastor, Ernesten_US
dc.contributor.authorde Lichtenberg, Casperen_US
dc.contributor.authorHussein, Ranaen_US
dc.contributor.authorPollock, Christopher J.en_US
dc.contributor.authorZhang, Miaoen_US
dc.contributor.authorStan, Claudiu A.en_US
dc.contributor.authorKroll, Thomasen_US
dc.contributor.authorFransson, Thomasen_US
dc.contributor.authorWeninger, Clemensen_US
dc.contributor.authorKubin, Markusen_US
dc.contributor.authorAller, Pierreen_US
dc.contributor.authorLassalle, Louiseen_US
dc.contributor.authorBräuer, Philippen_US
dc.contributor.authorMiller, Mitchell D.en_US
dc.contributor.authorAmin, Muhameden_US
dc.contributor.authorKoroidov, Sergeyen_US
dc.contributor.authorRoessler, Christian G.en_US
dc.contributor.authorAllaire, Marcen_US
dc.contributor.authorSierra, Raymond G.en_US
dc.contributor.authorDocker, Peter T.en_US
dc.contributor.authorGlownia, James M.en_US
dc.contributor.authorNelson, Silkeen_US
dc.contributor.authorKoglin, Jason E.en_US
dc.contributor.authorZhu, Dilingen_US
dc.contributor.authorChollet, Matthieuen_US
dc.contributor.authorSong, Sanghoonen_US
dc.contributor.authorLemke, Henriken_US
dc.contributor.authorLiang, Mengningen_US
dc.contributor.authorSokaras, Dimosthenisen_US
dc.contributor.authorAlonso-Mori, Robertoen_US
dc.contributor.authorZouni, Athinaen_US
dc.contributor.authorMessinger, Johannesen_US
dc.contributor.authorBergmann, Uween_US
dc.contributor.authorBoal, Amie K.en_US
dc.contributor.authorBollinger, J.Martin Jr.en_US
dc.contributor.authorKrebs, Carstenen_US
dc.contributor.authorHögbom, Martinen_US
dc.contributor.authorPhillips, George N.Jr.en_US
dc.contributor.authorVierstra,, Richard D.en_US
dc.contributor.authorSauter, Nicholas K.en_US
dc.contributor.authorOrville, Allen M.en_US
dc.contributor.authorKern, Janen_US
dc.contributor.authorYachandra, Vittal K.en_US
dc.contributor.authorYano, Junkoen_US
dc.date.accessioned2017-07-25T18:04:13Zen_US
dc.date.available2017-07-25T18:04:13Zen_US
dc.date.issued2017en_US
dc.description.abstractX-ray crystallography at X-ray free-electron laser sources is a powerful method for studying macromolecules at biologically relevant temperatures. Moreover, when combined with complementary techniques like X-ray emission spectroscopy, both global structures and chemical properties of metalloenzymes can be obtained concurrently, providing insights into the interplay between the protein structure and dynamics and the chemistry at an active site. The implementation of such a multimodal approach can be compromised by conflicting requirements to optimize each individual method. In particular, the method used for sample delivery greatly affects the data quality. We present here a robust way of delivering controlled sample amounts on demand using acoustic droplet ejection coupled with a conveyor belt drive that is optimized for crystallography and spectroscopy measurements of photochemical and chemical reactions over a wide range of time scales. Studies with photosystem II, the phytochrome photoreceptor, and ribonucleotide reductase R2 illustrate the power and versatility of this method.en_US
dc.identifier.citationFuller, Franklin D., Gul, Sheraz, Chatterjee, Ruchira, et al.. "Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers." <i>Nature Methods,</i> 14, (2017) Nature Publishing Group: 443-449. https://doi.org/10.1038/nmeth.4195.en_US
dc.identifier.digitalDrop-on-Demand_Sample_Deliveryen_US
dc.identifier.doihttps://doi.org/10.1038/nmeth.4195en_US
dc.identifier.urihttps://hdl.handle.net/1911/95219en_US
dc.language.isoengen_US
dc.publisherNature Publishing Groupen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Nature Publishing Group.en_US
dc.titleDrop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasersen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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