Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers

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dc.citation.firstpage443
dc.citation.journalTitleNature Methods
dc.citation.lastpage449
dc.citation.volumeNumber14
dc.contributor.authorFuller, Franklin D.
dc.contributor.authorGul, Sheraz
dc.contributor.authorChatterjee, Ruchira
dc.contributor.authorBurgie, E.Sethe
dc.contributor.authorYoung, Iris D.
dc.contributor.authorLebrette, Hugo
dc.contributor.authorSrinivas, Vivek
dc.contributor.authorBrewster, Aaron S.
dc.contributor.authorMichels-Clark, Tara
dc.contributor.authorClinger, Jonathan A.
dc.contributor.authorAndi, Babak
dc.contributor.authorIbrahim, Mohamed
dc.contributor.authorPastor, Ernest
dc.contributor.authorde Lichtenberg, Casper
dc.contributor.authorHussein, Rana
dc.contributor.authorPollock, Christopher J.
dc.contributor.authorZhang, Miao
dc.contributor.authorStan, Claudiu A.
dc.contributor.authorKroll, Thomas
dc.contributor.authorFransson, Thomas
dc.contributor.authorWeninger, Clemens
dc.contributor.authorKubin, Markus
dc.contributor.authorAller, Pierre
dc.contributor.authorLassalle, Louise
dc.contributor.authorBräuer, Philipp
dc.contributor.authorMiller, Mitchell D.
dc.contributor.authorAmin, Muhamed
dc.contributor.authorKoroidov, Sergey
dc.contributor.authorRoessler, Christian G.
dc.contributor.authorAllaire, Marc
dc.contributor.authorSierra, Raymond G.
dc.contributor.authorDocker, Peter T.
dc.contributor.authorGlownia, James M.
dc.contributor.authorNelson, Silke
dc.contributor.authorKoglin, Jason E.
dc.contributor.authorZhu, Diling
dc.contributor.authorChollet, Matthieu
dc.contributor.authorSong, Sanghoon
dc.contributor.authorLemke, Henrik
dc.contributor.authorLiang, Mengning
dc.contributor.authorSokaras, Dimosthenis
dc.contributor.authorAlonso-Mori, Roberto
dc.contributor.authorZouni, Athina
dc.contributor.authorMessinger, Johannes
dc.contributor.authorBergmann, Uwe
dc.contributor.authorBoal, Amie K.
dc.contributor.authorBollinger, J.Martin Jr.
dc.contributor.authorKrebs, Carsten
dc.contributor.authorHögbom, Martin
dc.contributor.authorPhillips, George N.Jr.
dc.contributor.authorVierstra,, Richard D.
dc.contributor.authorSauter, Nicholas K.
dc.contributor.authorOrville, Allen M.
dc.contributor.authorKern, Jan
dc.contributor.authorYachandra, Vittal K.
dc.contributor.authorYano, Junko
dc.date.accessioned2017-07-25T18:04:13Z
dc.date.available2017-07-25T18:04:13Z
dc.date.issued2017
dc.description.abstractX-ray crystallography at X-ray free-electron laser sources is a powerful method for studying macromolecules at biologically relevant temperatures. Moreover, when combined with complementary techniques like X-ray emission spectroscopy, both global structures and chemical properties of metalloenzymes can be obtained concurrently, providing insights into the interplay between the protein structure and dynamics and the chemistry at an active site. The implementation of such a multimodal approach can be compromised by conflicting requirements to optimize each individual method. In particular, the method used for sample delivery greatly affects the data quality. We present here a robust way of delivering controlled sample amounts on demand using acoustic droplet ejection coupled with a conveyor belt drive that is optimized for crystallography and spectroscopy measurements of photochemical and chemical reactions over a wide range of time scales. Studies with photosystem II, the phytochrome photoreceptor, and ribonucleotide reductase R2 illustrate the power and versatility of this method.
dc.identifier.citationFuller, Franklin D., Gul, Sheraz, Chatterjee, Ruchira, et al.. "Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers." <i>Nature Methods,</i> 14, (2017) Nature Publishing Group: 443-449. https://doi.org/10.1038/nmeth.4195.
dc.identifier.digitalDrop-on-Demand_Sample_Delivery
dc.identifier.doihttps://doi.org/10.1038/nmeth.4195
dc.identifier.urihttps://hdl.handle.net/1911/95219
dc.language.isoeng
dc.publisherNature Publishing Group
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Nature Publishing Group.
dc.titleDrop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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