Single molecule sequencing of the M13 virus genome without amplification

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dc.citation.articleNumbere0188181en_US
dc.citation.issueNumber12en_US
dc.citation.journalTitlePLoS ONEen_US
dc.citation.volumeNumber12en_US
dc.contributor.authorZhao, Luyangen_US
dc.contributor.authorDeng, Liweien_US
dc.contributor.authorLi, Gailingen_US
dc.contributor.authorJin, Huanen_US
dc.contributor.authorCai, Jinsenen_US
dc.contributor.authorShang, Huanen_US
dc.contributor.authorLi, Yanen_US
dc.contributor.authorWu, Haominen_US
dc.contributor.authorXu, Weibinen_US
dc.contributor.authorZeng, Lidongen_US
dc.contributor.authorZhang, Renlien_US
dc.contributor.authorZhao, Huanen_US
dc.contributor.authorWu, Pingen_US
dc.contributor.authorZhou, Zhiliangen_US
dc.contributor.authorZheng, Jiaoen_US
dc.contributor.authorEzanno, Pierreen_US
dc.contributor.authorYang, Andrew X.en_US
dc.contributor.authorYan, Qinen_US
dc.contributor.authorDeem, Michael W.en_US
dc.contributor.authorHe, Jiankuien_US
dc.date.accessioned2018-07-11T15:59:27Zen_US
dc.date.available2018-07-11T15:59:27Zen_US
dc.date.issued2017en_US
dc.description.abstractNext generation sequencing (NGS) has revolutionized life sciences research. However, GC bias and costly, time-intensive library preparation make NGS an ill fit for increasing sequencing demands in the clinic. A new class of third-generation sequencing platforms has arrived to meet this need, capable of directly measuring DNA and RNA sequences at the single-molecule level without amplification. Here, we use the new GenoCare single-molecule sequencing platform from Direct Genomics to sequence the genome of the M13 virus. Our platform detects single-molecule fluorescence by total internal reflection microscopy, with sequencing-by-synthesis chemistry. We sequenced the genome of M13 to a depth of 316x, with 100% coverage. We determined a consensus sequence accuracy of 100%. In contrast to GC bias inherent to NGS results, we demonstrated that our single-molecule sequencing method yields minimal GC bias.en_US
dc.identifier.citationZhao, Luyang, Deng, Liwei, Li, Gailing, et al.. "Single molecule sequencing of the M13 virus genome without amplification." <i>PLoS ONE,</i> 12, no. 12 (2017) Public Library of Science: https://doi.org/10.1371/journal.pone.0188181.en_US
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0188181en_US
dc.identifier.urihttps://hdl.handle.net/1911/102376en_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsThis is an open access article distributed under the terms of theﾠCreative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleSingle molecule sequencing of the M13 virus genome without amplificationen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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