BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo

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dc.citation.articleNumberbio058617en_US
dc.citation.issueNumber9en_US
dc.citation.journalTitleBiology Openen_US
dc.citation.volumeNumber10en_US
dc.contributor.authorChhabra, Sapnaen_US
dc.contributor.authorWarmflash, Aryehen_US
dc.date.accessioned2021-10-21T17:53:21Zen_US
dc.date.available2021-10-21T17:53:21Zen_US
dc.date.issued2021en_US
dc.description.abstractHuman embryonic stem cells (hESCs) possess an immense potential to generate clinically relevant cell types and unveil mechanisms underlying early human development. However, using hESCs for discovery or translation requires accurately identifying differentiated cell types through comparison with their in vivo counterparts. Here, we set out to determine the identity of much debated BMP-treated hESCs by comparing their transcriptome to recently published single cell transcriptomic data from early human embryos ( Xiang et al., 2020). Our analyses reveal several discrepancies in the published human embryo dataset, including misclassification of putative amnion, intermediate and inner cell mass cells. These misclassifications primarily resulted from similarities in pseudogene expression, highlighting the need to carefully consider gene lists when making comparisons between cell types. In the absence of a relevant human dataset, we utilized the recently published single cell transcriptome of the early post implantation monkey embryo to discern the identity of BMP-treated hESCs. Our results suggest that BMP-treated hESCs are transcriptionally more similar to amnion cells than trophectoderm cells in the monkey embryo. Together with prior studies, this result indicates that hESCs possess a unique ability to form mature trophectoderm subtypes via an amnion-like transcriptional state.This article has an associated First Person interview with the first author of the paper.en_US
dc.identifier.citationChhabra, Sapna and Warmflash, Aryeh. "BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo." <i>Biology Open,</i> 10, no. 9 (2021) The Company of Biologists: https://doi.org/10.1242/bio.058617.en_US
dc.identifier.digitalbio058617en_US
dc.identifier.doihttps://doi.org/10.1242/bio.058617en_US
dc.identifier.urihttps://hdl.handle.net/1911/111587en_US
dc.language.isoengen_US
dc.publisherThe Company of Biologistsen_US
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleBMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryoen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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