Controlled and Stable Patterning of Diverse Inorganic Nanocrystals on Crystalline Two-Dimensional Protein Arrays

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dc.citation.firstpage1063en_US
dc.citation.issueNumber13en_US
dc.citation.journalTitleBiochemistryen_US
dc.citation.lastpage1074en_US
dc.citation.volumeNumber60en_US
dc.contributor.authorMann, Victor R.en_US
dc.contributor.authorManea, Francescaen_US
dc.contributor.authorBorys, Nicholas J.en_US
dc.contributor.authorAjo-Franklin, Caroline M.en_US
dc.contributor.authorCohen, Bruce E.en_US
dc.date.accessioned2021-05-12T15:10:05Zen_US
dc.date.available2021-05-12T15:10:05Zen_US
dc.date.issued2021en_US
dc.description.abstractControlled patterning of nanoparticles on bioassemblies enables synthesis of complex materials for applications in optics, nanoelectronics, and sensing. Biomolecular self-assembly offers molecular control for engineering patterned nanomaterials, but current approaches have been limited in their ability to combine high nanoparticle coverage with generality that enables incorporation of multiple nanoparticle types. Here, we synthesize photonic materials on crystalline two-dimensional (2D) protein sheets using orthogonal bioconjugation reactions, organizing quantum dots (QDs), gold nanoparticles (AuNPs), and upconverting nanoparticles along the surface-layer (S-layer) protein SbsB from the extremophile Geobacillus stearothermophilus. We use electron and optical microscopy to show that isopeptide bond-forming SpyCatcher and SnoopCatcher systems enable the simultaneous and controlled conjugation of multiple types of nanoparticles (NPs) at high densities along the SbsB sheets. These NP conjugation reactions are orthogonal to each other and to Au–thiol bond formation, allowing tailorable nanoparticle combinations at sufficient labeling efficiencies to permit optical interactions between nanoparticles. Fluorescence lifetime imaging of SbsB sheets conjugated to QDs and AuNPs at distinct attachment sites shows spatially heterogeneous QD emission, with shorter radiative decays and brighter fluorescence arising from plasmonic enhancement at short interparticle distances. This specific, stable, and efficient conjugation of NPs to 2D protein sheets enables the exploration of interactions between pairs of nanoparticles at defined distances for the engineering of protein-based photonic nanomaterials.en_US
dc.identifier.citationMann, Victor R., Manea, Francesca, Borys, Nicholas J., et al.. "Controlled and Stable Patterning of Diverse Inorganic Nanocrystals on Crystalline Two-Dimensional Protein Arrays." <i>Biochemistry,</i> 60, no. 13 (2021) American Chemical Society: 1063-1074. https://doi.org/10.1021/acs.biochem.1c00032.en_US
dc.identifier.doihttps://doi.org/10.1021/acs.biochem.1c00032en_US
dc.identifier.urihttps://hdl.handle.net/1911/110508en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.en_US
dc.titleControlled and Stable Patterning of Diverse Inorganic Nanocrystals on Crystalline Two-Dimensional Protein Arraysen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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