Age-Related Differences in the Mouse Corneal Epithelial Transcriptome and Their Impact on Corneal Wound Healing

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dc.citation.articleNumber21en_US
dc.citation.issueNumber5en_US
dc.citation.journalTitleInvestigative Ophthalmology & Visual Scienceen_US
dc.citation.volumeNumber65en_US
dc.contributor.authorAbu-Romman, Anmaren_US
dc.contributor.authorScholand, Kaitlin K.en_US
dc.contributor.authorGovindarajan, Gowthamanen_US
dc.contributor.authorYu, Zhiyuanen_US
dc.contributor.authorPal-Ghosh, Sonalien_US
dc.contributor.authorStepp, Mary A.en_US
dc.contributor.authorde Paiva, Cintia S.en_US
dc.date.accessioned2024-08-02T13:32:09Zen_US
dc.date.available2024-08-02T13:32:09Zen_US
dc.date.issued2024en_US
dc.description.abstractAging is a risk factor for dry eye. We sought to identify changes in the aged mouse corneal epithelial transcriptome and determine how age affects corneal sensitivity, re-epithelialization, and barrier reformation after corneal debridement. Corneal epithelium of female C57BL/6J (B6) mice of different ages (2, 12, 18, and 24 months) was collected, RNA extracted, and bulk RNA sequencing performed. Cornea sensitivity was measured with an esthesiometer in 2- to 3-month-old, 12- to 13-month-old, 18- to 19-month-old, and 22- to 25-month-old female and male mice. The 2-month-old and 18-month-old female and male mice underwent unilateral corneal debridement using a blunt blade. Wound size and fluorescein staining were visualized and photographed at different time points, and a re-epithelialization rate curve was calculated. There were 157 differentially expressed genes in aged mice compared with young mice. Several pathways downregulated with age control cell migration, proteoglycan synthesis, and collagen trimerization, assembly, biosynthesis, and degradation. Male mice had decreased corneal sensitivity compared with female mice at 12 and 24 months of age. Aged mice, irrespective of sex, had delayed corneal re-epithelialization in the first 48 hours and worse corneal fluorescein staining intensity at day 14 than young mice. Aged corneal epithelium has an altered transcriptome. Aged mice regardless of sex heal more slowly and displayed more signs of corneal epithelial defects after wounding than young mice. These results indicate that aging significantly alters the corneal epithelium and its ability to coordinate healing.en_US
dc.identifier.citationAbu-Romman, A., Scholand, K. K., Govindarajan, G., Yu, Z., Pal-Ghosh, S., Stepp, M. A., & de Paiva, C. S. (2024). Age-Related Differences in the Mouse Corneal Epithelial Transcriptome and Their Impact on Corneal Wound Healing. Investigative Ophthalmology & Visual Science, 65(5), 21. https://doi.org/10.1167/iovs.65.5.21en_US
dc.identifier.digitali1552-5783-65-5-21_1715420740-47953en_US
dc.identifier.doihttps://doi.org/10.1167/iovs.65.5.21en_US
dc.identifier.urihttps://hdl.handle.net/1911/117570en_US
dc.language.isoengen_US
dc.publisherARVOen_US
dc.rightsExcept where otherwise noted, this work is licensed under a Creative Commons Attribution (CC BY) license.  Permission to reuse, publish, or reproduce the work beyond the terms of the license or beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleAge-Related Differences in the Mouse Corneal Epithelial Transcriptome and Their Impact on Corneal Wound Healingen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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