Photobleaching Lifetimes of Cyanine Fluorophores Used for Single Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems

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dc.citation.firstpage1075
dc.citation.issueNumber9
dc.citation.journalTitleChemBioChem
dc.citation.lastpage1080
dc.citation.volumeNumber14
dc.contributor.authorCooper, David
dc.contributor.authorUhm, Heui
dc.contributor.authorTauzin, Lawrence J.
dc.contributor.authorPoddar, Nitesh
dc.contributor.authorLandes, Christy F.
dc.date.accessioned2014-10-06T17:18:01Z
dc.date.available2014-10-06T17:18:01Z
dc.date.issued2013
dc.description.abstractWe investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single-molecule environment. We found that the glucose/glucose oxygen-scavenging solution in combination with redox-based photostabilization solutions yielded the longest average photobleaching lifetimes
dc.identifier.citationCooper, David, Uhm, Heui, Tauzin, Lawrence J., et al.. "Photobleaching Lifetimes of Cyanine Fluorophores Used for Single Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems." <i>ChemBioChem,</i> 14, no. 9 (2013) John Wiley & Sons, Inc.: 1075-1080. http://dx.doi.org/10.1002/cbic.201300030.
dc.identifier.doihttp://dx.doi.org/10.1002/cbic.201300030
dc.identifier.urihttps://hdl.handle.net/1911/77399
dc.language.isoeng
dc.publisherJohn Wiley & Sons, Inc.
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrightedby John Wiley & Sons Ltd.
dc.subject.keywordDNA
dc.subject.keywordfluorophore
dc.subject.keywordFRET
dc.subject.keywordphotobleach
dc.subject.keywordsingle-molecule studies
dc.titlePhotobleaching Lifetimes of Cyanine Fluorophores Used for Single Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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