A PEX5 missense allele preferentially disrupts PTS1 cargo import into Arabidopsis peroxisomes

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dc.citation.firstpage1en_US
dc.citation.issueNumber3en_US
dc.citation.journalTitlePlant Directen_US
dc.citation.lastpage13en_US
dc.citation.volumeNumber3en_US
dc.contributor.authorPatel, Khushali J.en_US
dc.contributor.authorKao, Yun-Tingen_US
dc.contributor.authorLlinas, Roxanna J.en_US
dc.contributor.authorBartel, Bonnieen_US
dc.contributor.orgBioSciencesen_US
dc.date.accessioned2019-04-08T16:01:37Zen_US
dc.date.available2019-04-08T16:01:37Zen_US
dc.date.issued3/20/2019en_US
dc.description.abstractThe sorting of eukaryotic proteins to various organellar destinations requires receptors that recognize cargo protein targeting signals and facilitate transport into the organelle. One such receptor is the peroxin PEX5, which recruits cytosolic cargo carrying a peroxisome‐targeting signal (PTS) type 1 (PTS1) for delivery into the peroxisomal lumen (matrix). In plants and mammals, PEX5 is also indirectly required for peroxisomal import of proteins carrying a PTS2 signal because PEX5 binds the PTS2 receptor, bringing the associated PTS2 cargo to the peroxisome along with PTS1 cargo. Despite PEX5 being the PTS1 cargo receptor, previously identified Arabidopsis pex5 mutants display either impairment of both PTS1 and PTS2 import or defects only in PTS2 import. Here, we report the first Arabidopsis pex5 mutant with an exclusive PTS1 import defect. In addition to markedly diminished GFP‐PTS1 import and decreased pex5‐2 protein accumulation, this pex5‐2 mutant shows typical peroxisome‐related defects, including inefficient β‐oxidation and reduced growth. Growth at reduced or elevated temperatures ameliorated or exacerbated pex5‐2 peroxisome‐related defects, respectively, without markedly changing pex5‐2 protein levels. In contrast to the diminished PTS1 import, PTS2 processing was only slightly impaired and PTS2‐GFP import appeared normal in pex5‐2. This finding suggests that even minor peroxisomal localization of the PTS1 protein DEG15, the PTS2‐processing protease, is sufficient to maintain robust PTS2 processing.en_US
dc.identifier.citationPatel, Khushali J., Kao, Yun-Ting, Llinas, Roxanna J., et al.. "A PEX5 missense allele preferentially disrupts PTS1 cargo import into Arabidopsis peroxisomes." <i>Plant Direct,</i> 3, no. 3 (2019) American Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd.: 1-13. https://doi.org/10.1002/pld3.128.en_US
dc.identifier.digitalPatel_et_al-2019-Plant_Directen_US
dc.identifier.doihttps://doi.org/10.1002/pld3.128en_US
dc.identifier.urihttps://hdl.handle.net/1911/105268en_US
dc.language.isoengen_US
dc.publisherAmerican Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd.en_US
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International (CC BY 4.0)en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordArabidopsis thalianaen_US
dc.subject.keywordperoxinen_US
dc.subject.keywordperoxisome importen_US
dc.subject.keywordperoxisome‐targeting signalen_US
dc.subject.keywordPEX5en_US
dc.titleA PEX5 missense allele preferentially disrupts PTS1 cargo import into Arabidopsis peroxisomesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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