Single molecule targeted sequencing for cancer gene mutation detection

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dc.citation.articleNumber26110en_US
dc.citation.journalTitleScientific Reportsen_US
dc.citation.volumeNumber6en_US
dc.contributor.authorGao, Yanen_US
dc.contributor.authorDeng, Liweien_US
dc.contributor.authorYan, Qinen_US
dc.contributor.authorGao, Yongqianen_US
dc.contributor.authorWu, Zengdingen_US
dc.contributor.authorCai, Jinsenen_US
dc.contributor.authorJi, Daoruien_US
dc.contributor.authorLi, Gailingen_US
dc.contributor.authorWu, Pingen_US
dc.contributor.authorJin, Huanen_US
dc.contributor.authorZhao, Luyangen_US
dc.contributor.authorLiu, Songen_US
dc.contributor.authorGe, Liangjinen_US
dc.contributor.authorDeem, Michael W.en_US
dc.contributor.authorHe, Jiankuien_US
dc.date.accessioned2016-11-10T22:23:40Zen_US
dc.date.available2016-11-10T22:23:40Zen_US
dc.date.issued2016en_US
dc.description.abstractWith the rapid decline in cost of sequencing, it is now affordable to examine multiple genes in a single disease-targeted clinical test using next generation sequencing. Current targeted sequencing methods require a separate step of targeted capture enrichment during sample preparation before sequencing. Although there are fast sample preparation methods available in market, the library preparation process is still relatively complicated for physicians to use routinely. Here, we introduced an amplification-free Single Molecule Targeted Sequencing (SMTS) technology, which combined targeted capture and sequencing in one step. We demonstrated that this technology can detect low-frequency mutations using artificially synthesized DNA sample. SMTS has several potential advantages, including simple sample preparation thus no biases and errors are introduced by PCR reaction. SMTS has the potential to be an easy and quick sequencing technology for clinical diagnosis such as cancer gene mutation detection, infectious disease detection, inherited condition screening and noninvasive prenatal diagnosis.en_US
dc.identifier.citationGao, Yan, Deng, Liwei, Yan, Qin, et al.. "Single molecule targeted sequencing for cancer gene mutation detection." <i>Scientific Reports,</i> 6, (2016) Springer Nature: http://dx.doi.org/10.1038/srep26110.en_US
dc.identifier.doihttp://dx.doi.org/10.1038/srep26110en_US
dc.identifier.urihttps://hdl.handle.net/1911/92702en_US
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the articleﾒs Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.titleSingle molecule targeted sequencing for cancer gene mutation detectionen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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