Polydopamine-assisted one-step immobilization of lipase on α-alumina membrane for fouling control in the treatment of oily wastewater

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dc.citation.articleNumber141516en_US
dc.citation.journalTitleChemical Engineering Journalen_US
dc.citation.volumeNumber459en_US
dc.contributor.authorMulinari, Jéssicaen_US
dc.contributor.authorAmbrosi, Alanen_US
dc.contributor.authorFeng, Yurenen_US
dc.contributor.authorHe, Zeen_US
dc.contributor.authorHuang, Xiaochuanen_US
dc.contributor.authorLi, Qilinen_US
dc.contributor.authorDi Luccio, Marcoen_US
dc.contributor.authorHotza, Dachamiren_US
dc.contributor.authorOliveira, J. Vladimiren_US
dc.contributor.orgNanotechnology-Enabled Water Treatment Center (NEWT)en_US
dc.date.accessioned2023-02-20T17:17:47Zen_US
dc.date.available2023-02-20T17:17:47Zen_US
dc.date.issued2023en_US
dc.description.abstractCovalent enzyme immobilization is generally a time-consuming and multistep procedure that uses toxic solvents and requires more than one chemical, making industrial upscaling unattractive. Using an aqueous polydopamine (PDA) solution for enzyme immobilization is a greener alternative. Usually, enzyme immobilization using PDA is performed in two steps: dopamine polymerization on the material surface followed by enzyme immobilization. A few recent studies applied a one-step strategy by mixing dopamine and enzyme in the coating solution, reducing the immobilization time, chemical consumption, and wastewater generation. This study compares the two-step and one-step approaches to immobilizing the lipase Eversa Transform 2.0 (ET2) on an α-alumina membrane. The one-step immobilization method achieved similar enzyme loading, membrane hydrolytic activity, and enzyme-specific activity to those of the two-step method. The ET2 immobilized using both strategies showed excellent fouling resistance and self-cleaning performance in oily wastewater filtration. The membrane modified by the one-step approach exhibited a lower reduction in pure water permeance after oil fouling (35%) and a higher permeance recovery (90%) than the one modified by the two-step method (40% and 74%, respectively). This better performance can be due to the higher hydrophilicity of the modified membrane and higher stability over reaction time shown by the enzyme immobilized by the one-step strategy. The higher stability can be attributed to more attachment points between the enzyme and PDA, increasing the enzyme rigidity and preventing conformational changes.en_US
dc.identifier.citationMulinari, Jéssica, Ambrosi, Alan, Feng, Yuren, et al.. "Polydopamine-assisted one-step immobilization of lipase on α-alumina membrane for fouling control in the treatment of oily wastewater." <i>Chemical Engineering Journal,</i> 459, (2023) Elsevier: https://doi.org/10.1016/j.cej.2023.141516.en_US
dc.identifier.doihttps://doi.org/10.1016/j.cej.2023.141516en_US
dc.identifier.urihttps://hdl.handle.net/1911/114462en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.en_US
dc.subject.keywordEversa transform 2.0en_US
dc.subject.keywordEnzyme immobilizationen_US
dc.subject.keywordCeramic membraneen_US
dc.subject.keywordReactive membraneen_US
dc.subject.keywordPolydopamineen_US
dc.subject.keywordOne-pot immobilizationen_US
dc.titlePolydopamine-assisted one-step immobilization of lipase on α-alumina membrane for fouling control in the treatment of oily wastewateren_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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