Line-scanning confocal microendoscope for nuclear morphometry imaging

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dc.citation.articleNumber116005en_US
dc.citation.issueNumber11en_US
dc.citation.journalTitleJournal of Biomedical Opticsen_US
dc.citation.volumeNumber22en_US
dc.contributor.authorTang, Yuboen_US
dc.contributor.authorCarns, Jenniferen_US
dc.contributor.authorRichards-Kortum, Rebecca R.en_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2017-12-21T18:21:51Zen_US
dc.date.available2017-12-21T18:21:51Zen_US
dc.date.issued2017en_US
dc.description.abstractFiber-optic endomicroscopy is a minimally invasive method to image cellular morphology in vivo. Using a coherent fiber bundle as an image relay, it allows additional imaging optics to be placed at the distal end of the fiber outside the body. In this research, we use this approach to demonstrate a compact, low-cost line-scanning confocal fluorescence microendoscope that can be constructed for <$5000 . Confocal imaging is enabled without the need for mechanical scanning by synchronizing a digital light projector with the rolling shutter of a CMOS camera. Its axial performance is characterized in comparison with a nonscanned high-resolution microendoscope. We validate the optical sectioning capability of the microendoscope by imaging a two-dimensional phantom and ex vivo mouse esophageal and colon tissues. Results show that optical sectioning using this approach improves visualization of nuclear morphometry and suggest that this low-cost line-scanning microendoscope can be used to evaluate various pathological conditions.en_US
dc.identifier.citationTang, Yubo, Carns, Jennifer and Richards-Kortum, Rebecca R.. "Line-scanning confocal microendoscope for nuclear morphometry imaging." <i>Journal of Biomedical Optics,</i> 22, no. 11 (2017) SPIE: https://doi.org/10.1117/1.JBO.22.11.116005.en_US
dc.identifier.digitalLine-scanning-confocal-microendoscopeen_US
dc.identifier.doihttps://doi.org/10.1117/1.JBO.22.11.116005en_US
dc.identifier.urihttps://hdl.handle.net/1911/98911en_US
dc.language.isoengen_US
dc.publisherSPIEen_US
dc.rightsPublished by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/en_US
dc.subject.keywordCMOS rolling shutteren_US
dc.subject.keyworddigital light projectoren_US
dc.subject.keywordline-scanningen_US
dc.subject.keywordlow-costen_US
dc.subject.keywordmicroendoscopeen_US
dc.subject.keywordoptical sectioningen_US
dc.titleLine-scanning confocal microendoscope for nuclear morphometry imagingen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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