Beyond Sequence: Internucleosomal Interactions Dominate Array Assembly

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dc.citation.firstpage10813en_US
dc.citation.issueNumber51en_US
dc.citation.journalTitleThe Journal of Physical Chemistry Ben_US
dc.citation.lastpage10821en_US
dc.citation.volumeNumber126en_US
dc.contributor.authorWang, Yaqingen_US
dc.contributor.authorStormberg, Tommyen_US
dc.contributor.authorHashemi, Mohtadinen_US
dc.contributor.authorKolomeisky, Anatoly B.en_US
dc.contributor.authorLyubchenko, Yuri L.en_US
dc.contributor.orgCenter for Theoretical Biological Physicsen_US
dc.date.accessioned2023-01-10T16:18:18Zen_US
dc.date.available2023-01-10T16:18:18Zen_US
dc.date.issued2022en_US
dc.description.abstractThe organization of the nucleosome array is a critical component of the chromatin assembly into higher order structure as well as its function. Here, we investigated the contributions of the DNA sequence and internucleosomal interactions on the organization of the nucleosomal arrays in compact structures using atomic force microscopy. We assembled nucleosomes on DNA substrates allowing for the formation of tetranucleosomes. We found that nucleosomes are capable of close positioning with no discernible space between them, even in the case of assembled dinucleosomes. This morphology of the array is in contrast with that observed for arrays assembled with repeats of the nucleosome positioning motifs separated by uniform spacers. Simulated assembly of tetranucleosomes by random placement along the substrates revealed that nucleosome array compaction is promoted by the interaction of the nucleosomes. We developed a theoretical model to account for the role of DNA sequence and internucleosomal interactions in the formation of the nucleosome structures. These findings suggest that, in the chromatin assembly, the affinity of the nucleosomes to the DNA sequence and the strengths of the internucleosomal interactions are the two major factors defining the compactness of the chromatin.en_US
dc.identifier.citationWang, Yaqing, Stormberg, Tommy, Hashemi, Mohtadin, et al.. "Beyond Sequence: Internucleosomal Interactions Dominate Array Assembly." <i>The Journal of Physical Chemistry B,</i> 126, no. 51 (2022) American Chemical Society: 10813-10821. https://doi.org/10.1021/acs.jpcb.2c05321.en_US
dc.identifier.doihttps://doi.org/10.1021/acs.jpcb.2c05321en_US
dc.identifier.urihttps://hdl.handle.net/1911/114233en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.en_US
dc.titleBeyond Sequence: Internucleosomal Interactions Dominate Array Assemblyen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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