Emergent genetic oscillations in a synthetic microbial consortium

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dc.citation.firstpage986en_US
dc.citation.issueNumber6251en_US
dc.citation.journalTitleScienceen_US
dc.citation.lastpage989en_US
dc.citation.volumeNumber349en_US
dc.contributor.authorChen, Yeen_US
dc.contributor.authorKim, Jae Kyoungen_US
dc.contributor.authorHirning, Andrew J.en_US
dc.contributor.authorJosić, Krešimiren_US
dc.contributor.authorBennett, Matthew R.en_US
dc.contributor.orgInstitute of Biosciences and Bioengineeringen_US
dc.date.accessioned2016-06-22T20:08:01Zen_US
dc.date.available2016-06-22T20:08:01Zen_US
dc.date.issued2015en_US
dc.description.abstractA challenge of synthetic biology is the creation of cooperative microbial systems that exhibit population-level behaviors. Such systems use cellular signaling mechanisms to regulate gene expression across multiple cell types. We describe the construction of a synthetic microbial consortium consisting of two distinct cell types—an "activator" strain and a "repressor" strain. These strains produced two orthogonal cell-signaling molecules that regulate gene expression within a synthetic circuit spanning both strains. The two strains generated emergent, population-level oscillations only when cultured together. Certain network topologies of the two-strain circuit were better at maintaining robust oscillations than others. The ability to program population-level dynamics through the genetic engineering of multiple cooperative strains points the way toward engineering complex synthetic tissues and organs with multiple cell types.en_US
dc.identifier.citationChen, Ye, Kim, Jae Kyoung, Hirning, Andrew J., et al.. "Emergent genetic oscillations in a synthetic microbial consortium." <i>Science,</i> 349, no. 6251 (2015) American Association for the Advancement of Science: 986-989. http://dx.doi.org/10.1126/science.aaa3794.en_US
dc.identifier.doihttp://dx.doi.org/10.1126/science.aaa3794en_US
dc.identifier.urihttps://hdl.handle.net/1911/90534en_US
dc.language.isoengen_US
dc.publisherAmerican Association for the Advancement of Scienceen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the ﾠAmerican Association for the Advancement of Science.en_US
dc.titleEmergent genetic oscillations in a synthetic microbial consortiumen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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