Assessing the impact of silicon nanowires on bacterial transformation and viability of Escherichia coli

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dc.citation.firstpage4906en_US
dc.citation.issueNumber24en_US
dc.citation.journalTitleJournal of Materials Chemistry Ben_US
dc.citation.lastpage4914en_US
dc.citation.volumeNumber9en_US
dc.contributor.authorBecce, Micheleen_US
dc.contributor.authorKlöckner, Annaen_US
dc.contributor.authorHiggins, Stuart G.en_US
dc.contributor.authorPenders, Jelleen_US
dc.contributor.authorHachim, Danielen_US
dc.contributor.authorBashor, Caleb J.en_US
dc.contributor.authorEdwards, Andrew M.en_US
dc.contributor.authorStevens, Molly M.en_US
dc.date.accessioned2021-07-08T13:46:00Zen_US
dc.date.available2021-07-08T13:46:00Zen_US
dc.date.issued2021en_US
dc.description.abstractWe investigated the biomaterial interface between the bacteria Escherichia coli DH5α and silicon nanowire patterned surfaces. We optimised the engineering of silicon nanowire coated surfaces using metal-assisted chemical etching. Using a combination of focussed ion beam scanning electron microscopy, and cell viability and transformation assays, we found that with increasing interfacing force, cell viability decreases, as a result of increasing cell rupture. However, despite this aggressive interfacing regime, a proportion of the bacterial cell population remains viable. We found that the silicon nanowires neither resulted in complete loss of cell viability nor partial membrane disruption and corresponding DNA plasmid transformation. Critically, assay choice was observed to be important, as a reduction-based metabolic reagent was found to yield false-positive results on the silicon nanowire substrate. We discuss the implications of these results for the future design and assessment of bacteria–nanostructure interfacing experiments.en_US
dc.identifier.citationBecce, Michele, Klöckner, Anna, Higgins, Stuart G., et al.. "Assessing the impact of silicon nanowires on bacterial transformation and viability of Escherichia coli." <i>Journal of Materials Chemistry B,</i> 9, no. 24 (2021) Royal Society of Chemistry: 4906-4914. https://doi.org/10.1039/D0TB02762F.en_US
dc.identifier.digitald0tb02762fen_US
dc.identifier.doihttps://doi.org/10.1039/D0TB02762Fen_US
dc.identifier.urihttps://hdl.handle.net/1911/110998en_US
dc.language.isoengen_US
dc.publisherRoyal Society of Chemistryen_US
dc.rightsThis Open Access Article is licensed under a Creative Commons Attribution 3.0 Unported Licenceen_US
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/en_US
dc.titleAssessing the impact of silicon nanowires on bacterial transformation and viability of Escherichia colien_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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