Assessing the impact of silicon nanowires on bacterial transformation and viability of Escherichia coli

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dc.citation.firstpage4906
dc.citation.issueNumber24
dc.citation.journalTitleJournal of Materials Chemistry B
dc.citation.lastpage4914
dc.citation.volumeNumber9
dc.contributor.authorBecce, Michele
dc.contributor.authorKlöckner, Anna
dc.contributor.authorHiggins, Stuart G.
dc.contributor.authorPenders, Jelle
dc.contributor.authorHachim, Daniel
dc.contributor.authorBashor, Caleb J.
dc.contributor.authorEdwards, Andrew M.
dc.contributor.authorStevens, Molly M.
dc.date.accessioned2021-07-08T13:46:00Z
dc.date.available2021-07-08T13:46:00Z
dc.date.issued2021
dc.description.abstractWe investigated the biomaterial interface between the bacteria Escherichia coli DH5α and silicon nanowire patterned surfaces. We optimised the engineering of silicon nanowire coated surfaces using metal-assisted chemical etching. Using a combination of focussed ion beam scanning electron microscopy, and cell viability and transformation assays, we found that with increasing interfacing force, cell viability decreases, as a result of increasing cell rupture. However, despite this aggressive interfacing regime, a proportion of the bacterial cell population remains viable. We found that the silicon nanowires neither resulted in complete loss of cell viability nor partial membrane disruption and corresponding DNA plasmid transformation. Critically, assay choice was observed to be important, as a reduction-based metabolic reagent was found to yield false-positive results on the silicon nanowire substrate. We discuss the implications of these results for the future design and assessment of bacteria–nanostructure interfacing experiments.
dc.identifier.citationBecce, Michele, Klöckner, Anna, Higgins, Stuart G., et al.. "Assessing the impact of silicon nanowires on bacterial transformation and viability of Escherichia coli." <i>Journal of Materials Chemistry B,</i> 9, no. 24 (2021) Royal Society of Chemistry: 4906-4914. https://doi.org/10.1039/D0TB02762F.
dc.identifier.digitald0tb02762f
dc.identifier.doihttps://doi.org/10.1039/D0TB02762F
dc.identifier.urihttps://hdl.handle.net/1911/110998
dc.language.isoeng
dc.publisherRoyal Society of Chemistry
dc.rightsThis Open Access Article is licensed under a Creative Commons Attribution 3.0 Unported Licence
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.titleAssessing the impact of silicon nanowires on bacterial transformation and viability of Escherichia coli
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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