Rational engineering of a modular bacterial CRISPR–Cas activation platform with expanded target range

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dc.citation.firstpage4793
dc.citation.issueNumber8
dc.citation.journalTitleNucleic Acids Research
dc.citation.lastpage4802
dc.citation.volumeNumber49
dc.contributor.authorVillegas Kcam, Maria Claudia
dc.contributor.authorTsong, Annette J
dc.contributor.authorChappell, James
dc.date.accessioned2021-06-10T17:36:54Z
dc.date.available2021-06-10T17:36:54Z
dc.date.issued2021
dc.description.abstractCRISPR–Cas activator (CRISPRa) systems that selectively turn on transcription of a target gene are a potentially transformative technology for programming cellular function. While in eukaryotes versatile CRISPRa systems exist, in bacteria these systems suffer from a limited ability to activate different genes due to strict distance-dependent requirements of functional target binding sites, and require greater customization to optimize performance in different genetic and cellular contexts. To address this, we apply a rational protein engineering approach to create a new CRISPRa platform that is highly modular to allow for easy customization and has increased targeting flexibility through harnessing engineered Cas proteins. We first demonstrate that transcription activation domains can be recruited by CRISPR–Cas through noncovalent protein-protein interactions, which allows each component to be encoded on separate and easily interchangeable plasmid elements. We then exploit this modularity to rapidly screen a library of different activation domains, creating new systems with distinct regulatory properties. Furthermore, we demonstrate that by harnessing a library of circularly permuted Cas proteins, we can create CRISPRa systems that have different target binding site requirements, which together, allow for expanded target range.
dc.description.sponsorshipFondren Library Open Access Author Fund
dc.identifier.citationVillegas Kcam, Maria Claudia, Tsong, Annette J and Chappell, James. "Rational engineering of a modular bacterial CRISPR–Cas activation platform with expanded target range." <i>Nucleic Acids Research,</i> 49, no. 8 (2021) Oxford University Press: 4793-4802. https://doi.org/10.1093/nar/gkab211.
dc.identifier.digitalgkab211
dc.identifier.doihttps://doi.org/10.1093/nar/gkab211
dc.identifier.urihttps://hdl.handle.net/1911/110718
dc.language.isoeng
dc.publisherOxford University Press
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/),
dc.titleRational engineering of a modular bacterial CRISPR–Cas activation platform with expanded target range
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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