A viable Arabidopsis pex13 missense allele confers severe peroxisomal defects and decreases PEX5 association with peroxisomes

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dc.citation.firstpage201
dc.citation.issueNumber1
dc.citation.journalTitlePlant Molecular Biology
dc.citation.lastpage214
dc.citation.volumeNumber86
dc.contributor.authorWoodward, Andrew W.
dc.contributor.authorFleming, Wendell A.
dc.contributor.authorBurkhart, Sarah E.
dc.contributor.authorRatzel, Sarah E.
dc.contributor.authorBjornson, Marta
dc.contributor.authorBartel, Bonnie
dc.date.accessioned2015-10-28T19:04:03Z
dc.date.available2015-10-28T19:04:03Z
dc.date.issued2014
dc.description.abstractPeroxisomes are organelles that catabolize fatty acids and compartmentalize other oxidative metabolic processes in eukaryotes. Using a forward-genetic screen designed to recover severe peroxisome-defective mutants, we isolated a viable allele of the peroxisome biogenesis gene PEX13 with striking peroxisomal defects. The pex13-4 mutant requires an exogenous source of fixed carbon for pre-photosynthetic development and is resistant to the protoauxin indole-3-butyric acid. Delivery of peroxisome-targeted matrix proteins depends on the PEX5 receptor docking with PEX13 at the peroxisomal membrane, and we found severely reduced import of matrix proteins and less organelle-associated PEX5 in pex13-4 seedlings. Moreover, pex13-4 physiological and molecular defects were partially ameliorated when PEX5 was overexpressed, suggesting that PEX5 docking is partially compromised in this mutant and can be improved by increasing PEX5 levels. Because previously described Arabidopsis pex13 alleles either are lethal or confer only subtle defects, the pex13-4 mutant provides valuable insight into plant peroxisome receptor docking and matrix protein import.
dc.identifier.citationWoodward, Andrew W., Fleming, Wendell A., Burkhart, Sarah E., et al.. "A viable Arabidopsis pex13 missense allele confers severe peroxisomal defects and decreases PEX5 association with peroxisomes." <i>Plant Molecular Biology,</i> 86, no. 1 (2014) Springer: 201-214. http://dx.doi.org/10.1007/s11103-014-0223-8.
dc.identifier.doihttp://dx.doi.org/10.1007/s11103-014-0223-8
dc.identifier.urihttps://hdl.handle.net/1911/81941
dc.language.isoeng
dc.publisherSpringer
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Springer.
dc.subject.keywordperoxisome
dc.subject.keywordorganelle biogenesis
dc.subject.keywordsubcellular targeting
dc.titleA viable Arabidopsis pex13 missense allele confers severe peroxisomal defects and decreases PEX5 association with peroxisomes
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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