A viable Arabidopsis pex13 missense allele confers severe peroxisomal defects and decreases PEX5 association with peroxisomes

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dc.citation.firstpage201en_US
dc.citation.issueNumber1en_US
dc.citation.journalTitlePlant Molecular Biologyen_US
dc.citation.lastpage214en_US
dc.citation.volumeNumber86en_US
dc.contributor.authorWoodward, Andrew W.en_US
dc.contributor.authorFleming, Wendell A.en_US
dc.contributor.authorBurkhart, Sarah E.en_US
dc.contributor.authorRatzel, Sarah E.en_US
dc.contributor.authorBjornson, Martaen_US
dc.contributor.authorBartel, Bonnieen_US
dc.date.accessioned2015-10-28T19:04:03Zen_US
dc.date.available2015-10-28T19:04:03Zen_US
dc.date.issued2014en_US
dc.description.abstractPeroxisomes are organelles that catabolize fatty acids and compartmentalize other oxidative metabolic processes in eukaryotes. Using a forward-genetic screen designed to recover severe peroxisome-defective mutants, we isolated a viable allele of the peroxisome biogenesis gene PEX13 with striking peroxisomal defects. The pex13-4 mutant requires an exogenous source of fixed carbon for pre-photosynthetic development and is resistant to the protoauxin indole-3-butyric acid. Delivery of peroxisome-targeted matrix proteins depends on the PEX5 receptor docking with PEX13 at the peroxisomal membrane, and we found severely reduced import of matrix proteins and less organelle-associated PEX5 in pex13-4 seedlings. Moreover, pex13-4 physiological and molecular defects were partially ameliorated when PEX5 was overexpressed, suggesting that PEX5 docking is partially compromised in this mutant and can be improved by increasing PEX5 levels. Because previously described Arabidopsis pex13 alleles either are lethal or confer only subtle defects, the pex13-4 mutant provides valuable insight into plant peroxisome receptor docking and matrix protein import.en_US
dc.identifier.citationWoodward, Andrew W., Fleming, Wendell A., Burkhart, Sarah E., et al.. "A viable Arabidopsis pex13 missense allele confers severe peroxisomal defects and decreases PEX5 association with peroxisomes." <i>Plant Molecular Biology,</i> 86, no. 1 (2014) Springer: 201-214. http://dx.doi.org/10.1007/s11103-014-0223-8.en_US
dc.identifier.doihttp://dx.doi.org/10.1007/s11103-014-0223-8en_US
dc.identifier.urihttps://hdl.handle.net/1911/81941en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Springer.en_US
dc.subject.keywordperoxisomeen_US
dc.subject.keywordorganelle biogenesisen_US
dc.subject.keywordsubcellular targetingen_US
dc.titleA viable Arabidopsis pex13 missense allele confers severe peroxisomal defects and decreases PEX5 association with peroxisomesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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