A spheroid toxicity assay using magnetic 3D bioprinting and real-time mobile device-based imaging

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dc.citation.articleNumber13987
dc.citation.journalTitleScientific Reports
dc.citation.volumeNumber5
dc.contributor.authorTseng, Hubert
dc.contributor.authorGage, Jacob A.
dc.contributor.authorShen, Tsaiwei
dc.contributor.authorHaisler, William L.
dc.contributor.authorNeeley, Shane K.
dc.contributor.authorShiao, Sue
dc.contributor.authorChen, Jianbo
dc.contributor.authorDesai, Pujan K.
dc.contributor.authorLiao, Angela
dc.contributor.authorHebel, Chris
dc.contributor.authorRaphael, Robert M.
dc.contributor.authorBecker, Jeanne L.
dc.contributor.authorSouza, Glauco R.
dc.date.accessioned2017-05-12T15:04:32Z
dc.date.available2017-05-12T15:04:32Z
dc.date.issued2015
dc.description.abstractAn ongoing challenge in biomedical research is the search for simple, yet robust assays using 3D cell cultures for toxicity screening. This study addresses that challenge with a novel spheroid assay, wherein spheroids, formed by magnetic 3D bioprinting, contract immediately as cells rearrange and compact the spheroid in relation to viability and cytoskeletal organization. Thus, spheroid size can be used as a simple metric for toxicity. The goal of this study was to validate spheroid contraction as a cytotoxic endpoint using 3T3 fibroblasts in response to 5 toxic compounds (all-trans retinoic acid, dexamethasone, doxorubicin, 5′-fluorouracil, forskolin), sodium dodecyl sulfate (+control), and penicillin-G (−control). Real-time imaging was performed with a mobile device to increase throughput and efficiency. All compounds but penicillin-G significantly slowed contraction in a dose-dependent manner (Z’ = 0.88). Cells in 3D were more resistant to toxicity than cells in 2D, whose toxicity was measured by the MTT assay. Fluorescent staining and gene expression profiling of spheroids confirmed these findings. The results of this study validate spheroid contraction within this assay as an easy, biologically relevant endpoint for high-throughput compound screening in representative 3D environments.
dc.identifier.citationTseng, Hubert, Gage, Jacob A., Shen, Tsaiwei, et al.. "A spheroid toxicity assay using magnetic 3D bioprinting and real-time mobile device-based imaging." <i>Scientific Reports,</i> 5, (2015) Springer Nature: http://dx.doi.org/10.1038/srep13987.
dc.identifier.doihttp://dx.doi.org/10.1038/srep13987
dc.identifier.urihttps://hdl.handle.net/1911/94234
dc.language.isoeng
dc.publisherSpringer Nature
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the articleﾒs Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleA spheroid toxicity assay using magnetic 3D bioprinting and real-time mobile device-based imaging
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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