Oxime as a general photocage for the design of visible light photo-activatable fluorophores

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dc.citation.firstpage15572
dc.citation.journalTitleChemical Science
dc.citation.lastpage15580
dc.citation.volumeNumber12
dc.contributor.authorWang, Lushun
dc.contributor.authorWang, Shichao
dc.contributor.authorTang, Juan
dc.contributor.authorEspinoza, Vanessa B.
dc.contributor.authorLoredo, Axel
dc.contributor.authorTian, Zeru
dc.contributor.authorWeisman, R. Bruce
dc.contributor.authorXiao, Han
dc.date.accessioned2022-01-07T16:17:20Z
dc.date.available2022-01-07T16:17:20Z
dc.date.issued2021
dc.description.abstractPhotoactivatable fluorophores have been widely used for tracking molecular and cellular dynamics with subdiffraction resolution. In this work, we have prepared a series of photoactivatable probes using the oxime moiety as a new class of photolabile caging group in which the photoactivation process is mediated by a highly efficient photodeoximation reaction. Incorporation of the oxime caging group into fluorophores results in loss of fluorescence. Upon light irradiation in the presence of air, the oxime-caged fluorophores are oxidized to their carbonyl derivatives, restoring strong fluorophore fluorescence. To demonstrate the utility of these oxime-caged fluorophores, we have created probes that target different organelles for live-cell confocal imaging. We also carried out photoactivated localization microscopy (PALM) imaging under physiological conditions using low-power light activation in the absence of cytotoxic additives. Our studies show that oximes represent a new class of visible-light photocages that can be widely used for cellular imaging, sensing, and photo-controlled molecular release.
dc.identifier.citationWang, Lushun, Wang, Shichao, Tang, Juan, et al.. "Oxime as a general photocage for the design of visible light photo-activatable fluorophores." <i>Chemical Science,</i> 12, (2021) Royal Society of Chemistry: 15572-15580. https://doi.org/10.1039/D1SC05351E.
dc.identifier.digitald1sc05351e
dc.identifier.doihttps://doi.org/10.1039/D1SC05351E
dc.identifier.urihttps://hdl.handle.net/1911/111914
dc.language.isoeng
dc.publisherRoyal Society of Chemistry
dc.rightsThis Open Access Article is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported Licence
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/
dc.titleOxime as a general photocage for the design of visible light photo-activatable fluorophores
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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