Intravital microscopy for evaluating tumor perfusion of nanoparticles exposed to non-invasive radiofrequency electric fields

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dc.citation.articleNumber5
dc.citation.journalTitleCancer Nanotechnology
dc.citation.volumeNumber7
dc.contributor.authorLapin, Norman A.
dc.contributor.authorKrzykawska-Serda, Martyna
dc.contributor.authorWare, Matthew J.
dc.contributor.authorCurley, Steven A.
dc.contributor.authorCorr, Stuart J.
dc.date.accessioned2017-05-22T21:48:43Z
dc.date.available2017-05-22T21:48:43Z
dc.date.issued2016
dc.description.abstractPoor biodistribution and accumulation of chemotherapeutics in tumors due to limitations on diffusive transport and high intra-tumoral pressures (Jain RK, Nat Med. 7(9):987–989, 2001) have prompted the investigation of adjunctive therapies to improve treatment outcomes. Hyperthermia has been widely applied in attempts to meet this need, but it is limited in its ability to reach tumors in deeply located body regions. High-intensity radiofrequency (RF) electric fields have the potential to overcome such barriers enhancing delivery and extravasation of chemotherapeutics. However, due to factors, including tumor heterogeneity and lack of kinetic information, there is insufficient understanding of time-resolved interaction between RF fields and tumor vasculature, drug molecules and nanoparticle (NP) vectors. Intravital microscopy (IVM) provides time-resolved high-definition images of specific tumor microenvironments, overcoming heterogeneity issues, and can be integrated with a portable RF device to enable detailed observation over time of the effects of the RF field on kinetics and biodistribution at the microvascular level. Herein, we provide a protocol describing the safe integration of IVM with a high-powered non-invasive RF field applied to 4T1 orthotopic breast tumors in live mice. Results show increased perfusion of NPs in microvasculature upon RF hyperthermia treatment and increased perfusion, release and spreading of injected reagents preferentially in irregular vessels during RF exposure.
dc.identifier.citationLapin, Norman A., Krzykawska-Serda, Martyna, Ware, Matthew J., et al.. "Intravital microscopy for evaluating tumor perfusion of nanoparticles exposed to non-invasive radiofrequency electric fields." <i>Cancer Nanotechnology,</i> 7, (2016) Springer: https://doi.org/10.1186/s12645-016-0016-7.
dc.identifier.doihttps://doi.org/10.1186/s12645-016-0016-7
dc.identifier.urihttps://hdl.handle.net/1911/94342
dc.language.isoeng
dc.publisherSpringer
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subject.keywordcancer
dc.subject.keywordhyperthermia
dc.subject.keywordintravital microscopy
dc.subject.keywordquantum dots
dc.subject.keywordradiofrequency fields
dc.subject.keywordvasculature
dc.titleIntravital microscopy for evaluating tumor perfusion of nanoparticles exposed to non-invasive radiofrequency electric fields
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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