Three-dimensional (3D) culture of bone-derived human 786-O renal cell carcinoma retains relevant clinical characteristics of bone metastases

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dc.citation.firstpage89en_US
dc.citation.issueNumber1en_US
dc.citation.journalTitleCancer Lettersen_US
dc.citation.lastpage95en_US
dc.citation.volumeNumber365en_US
dc.contributor.authorPan, Tianhongen_US
dc.contributor.authorFong, Eliza L.S.en_US
dc.contributor.authorMartinez, Marianeen_US
dc.contributor.authorHarrington, Daniel Antonen_US
dc.contributor.authorLin, Sue-Hwaen_US
dc.contributor.authorFarach-Carson, Mary C.en_US
dc.contributor.authorSatcher, Robert L.en_US
dc.date.accessioned2016-10-21T17:01:00Zen_US
dc.date.available2016-10-21T17:01:00Zen_US
dc.date.issued2015en_US
dc.description.abstractBone metastases from renal cell carcinoma (RCC) are typically lytic, destructive, and resistant to treatment regimens. Current in vitro models for studying metastasis introduce artifacts that limit their usefulness. Many features of tumors growing in bone are lost when human RCC cells are cultured in two-dimensional (2D) plastic substrata. In this study, we established that RCC spheroids, consisting of aggregates of cells, can be grown in a three-dimensional (3D) hyaluronate hydrogel-based culture system. The bone-derived human 786-O RCC subline proliferated and survived long term in these hydrogels. Additionally, RCC spheroids in 3D hydrogels demonstrated lower proliferation rates than their counterparts grown in 2D. Overall, gene expression patterns of RCC spheroids in 3D more closely mimicked those observed in vivo than did those of cells grown in 2D. Of particular importance, selected adhesion molecules, angiogenesis factors, and osteolytic factors that have been shown to be involved in RCC bone metastasis were found to be expressed at higher levels in 3D than in 2D cultures. We propose that the 3D culture system provides an improved platform for RCC bone metastasis studies compared with 2D systems.en_US
dc.identifier.citationPan, Tianhong, Fong, Eliza L.S., Martinez, Mariane, et al.. "Three-dimensional (3D) culture of bone-derived human 786-O renal cell carcinoma retains relevant clinical characteristics of bone metastases." <i>Cancer Letters,</i> 365, no. 1 (2015) Elsevier: 89-95. http://dx.doi.org/10.1016/j.canlet.2015.05.019.en_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.canlet.2015.05.019en_US
dc.identifier.urihttps://hdl.handle.net/1911/91973en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.en_US
dc.subject.keywordbone metastasisen_US
dc.subject.keywordhyaluronic aciden_US
dc.subject.keywordhydrogelen_US
dc.subject.keywordrenal cell carcinomaen_US
dc.subject.keywordthree dimensional cultureen_US
dc.titleThree-dimensional (3D) culture of bone-derived human 786-O renal cell carcinoma retains relevant clinical characteristics of bone metastasesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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