Three-dimensional (3D) culture of bone-derived human 786-O renal cell carcinoma retains relevant clinical characteristics of bone metastases

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dc.citation.firstpage89
dc.citation.issueNumber1
dc.citation.journalTitleCancer Letters
dc.citation.lastpage95
dc.citation.volumeNumber365
dc.contributor.authorPan, Tianhong
dc.contributor.authorFong, Eliza L.S.
dc.contributor.authorMartinez, Mariane
dc.contributor.authorHarrington, Daniel Anton
dc.contributor.authorLin, Sue-Hwa
dc.contributor.authorFarach-Carson, Mary C.
dc.contributor.authorSatcher, Robert L.
dc.date.accessioned2016-10-21T17:01:00Z
dc.date.available2016-10-21T17:01:00Z
dc.date.issued2015
dc.description.abstractBone metastases from renal cell carcinoma (RCC) are typically lytic, destructive, and resistant to treatment regimens. Current in vitro models for studying metastasis introduce artifacts that limit their usefulness. Many features of tumors growing in bone are lost when human RCC cells are cultured in two-dimensional (2D) plastic substrata. In this study, we established that RCC spheroids, consisting of aggregates of cells, can be grown in a three-dimensional (3D) hyaluronate hydrogel-based culture system. The bone-derived human 786-O RCC subline proliferated and survived long term in these hydrogels. Additionally, RCC spheroids in 3D hydrogels demonstrated lower proliferation rates than their counterparts grown in 2D. Overall, gene expression patterns of RCC spheroids in 3D more closely mimicked those observed in vivo than did those of cells grown in 2D. Of particular importance, selected adhesion molecules, angiogenesis factors, and osteolytic factors that have been shown to be involved in RCC bone metastasis were found to be expressed at higher levels in 3D than in 2D cultures. We propose that the 3D culture system provides an improved platform for RCC bone metastasis studies compared with 2D systems.
dc.identifier.citationPan, Tianhong, Fong, Eliza L.S., Martinez, Mariane, et al.. "Three-dimensional (3D) culture of bone-derived human 786-O renal cell carcinoma retains relevant clinical characteristics of bone metastases." <i>Cancer Letters,</i> 365, no. 1 (2015) Elsevier: 89-95. http://dx.doi.org/10.1016/j.canlet.2015.05.019.
dc.identifier.doihttp://dx.doi.org/10.1016/j.canlet.2015.05.019
dc.identifier.urihttps://hdl.handle.net/1911/91973
dc.language.isoeng
dc.publisherElsevier
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.subject.keywordbone metastasis
dc.subject.keywordhyaluronic acid
dc.subject.keywordhydrogel
dc.subject.keywordrenal cell carcinoma
dc.subject.keywordthree dimensional culture
dc.titleThree-dimensional (3D) culture of bone-derived human 786-O renal cell carcinoma retains relevant clinical characteristics of bone metastases
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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