Confocal fluorescence microscopy for rapid evaluation of invasive tumor cellularity of inflammatory breast carcinoma core needle biopsies

Simple item page
dc.citation.journalTitleBreast Cancer Research and Treatment
dc.contributor.authorDobbs, Jessica
dc.contributor.authorKrishnamurthy, Savitri
dc.contributor.authorKyrish, Matthew
dc.contributor.authorBenveniste, Ana Paula
dc.contributor.authorYang, Wei
dc.contributor.authorRichards-Kortum, Rebecca
dc.date.accessioned2014-12-01T17:26:01Z
dc.date.available2014-12-01T17:26:01Z
dc.date.issued2014
dc.description.abstractTissue sampling is a problematic issue for inflammatory breast carcinoma, and immediate evaluation following core needle biopsy is needed to evaluate specimen adequacy. We sought to determine if confocal fluorescence microscopy provides sufficient resolution to evaluate specimen adequacy by comparing invasive tumor cellularity estimated from standard histologic images to invasive tumor cellularity estimated from confocal images of breast core needle biopsy specimens. Grayscale confocal fluorescence images of breast core needle biopsy specimens were acquired following proflavine application. A breast-dedicated pathologist evaluated invasive tumor cellularity in histologic images with hematoxylin and eosin staining and in grayscale and false-colored confocal images of cores. Agreement between cellularity estimates was quantified using a kappa coefficient. 23 cores from 23 patients with suspected inflammatory breast carcinoma were imaged. Confocal images were acquired in an average of less than 2 min per core. Invasive tumor cellularity estimated from histologic and grayscale confocal images showed moderate agreement by kappa coefficient: κ = 0.48 ± 0.09 (p < 0.001). Grayscale confocal images require less than 2 min for acquisition and allow for evaluation of invasive tumor cellularity in breast core needle biopsy specimens with moderate agreement to histologic images. We show that confocal fluorescence microscopy can be performed immediately following specimen acquisition and could indicate the need for additional biopsies at the initial visit.
dc.identifier.citationDobbs, Jessica, Krishnamurthy, Savitri, Kyrish, Matthew, et al.. "Confocal fluorescence microscopy for rapid evaluation of invasive tumor cellularity of inflammatory breast carcinoma core needle biopsies." <i>Breast Cancer Research and Treatment,</i> (2014) Springer: http://dx.doi.org/10.1007/s10549-014-3182-5.
dc.identifier.doihttp://dx.doi.org/10.1007/s10549-014-3182-5
dc.identifier.urihttps://hdl.handle.net/1911/78536
dc.language.isoeng
dc.publisherSpringer
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subject.keywordinflammatory breast cancer
dc.subject.keywordcore needle biopsy
dc.subject.keywordconfocal fluorescence microscopy
dc.subject.keywordtissue sampling
dc.titleConfocal fluorescence microscopy for rapid evaluation of invasive tumor cellularity of inflammatory breast carcinoma core needle biopsies
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Confocal-fluorescence.pdf
Size:
2.17 MB
Format:
Adobe Portable Document Format
Download
Collections
Faculty Publications
Bioengineering Publications