Confocal fluorescence microscopy for rapid evaluation of invasive tumor cellularity of inflammatory breast carcinoma core needle biopsies

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dc.citation.journalTitleBreast Cancer Research and Treatmenten_US
dc.contributor.authorDobbs, Jessicaen_US
dc.contributor.authorKrishnamurthy, Savitrien_US
dc.contributor.authorKyrish, Matthewen_US
dc.contributor.authorBenveniste, Ana Paulaen_US
dc.contributor.authorYang, Weien_US
dc.contributor.authorRichards-Kortum, Rebeccaen_US
dc.date.accessioned2014-12-01T17:26:01Zen_US
dc.date.available2014-12-01T17:26:01Zen_US
dc.date.issued2014en_US
dc.description.abstractTissue sampling is a problematic issue for inflammatory breast carcinoma, and immediate evaluation following core needle biopsy is needed to evaluate specimen adequacy. We sought to determine if confocal fluorescence microscopy provides sufficient resolution to evaluate specimen adequacy by comparing invasive tumor cellularity estimated from standard histologic images to invasive tumor cellularity estimated from confocal images of breast core needle biopsy specimens. Grayscale confocal fluorescence images of breast core needle biopsy specimens were acquired following proflavine application. A breast-dedicated pathologist evaluated invasive tumor cellularity in histologic images with hematoxylin and eosin staining and in grayscale and false-colored confocal images of cores. Agreement between cellularity estimates was quantified using a kappa coefficient. 23 cores from 23 patients with suspected inflammatory breast carcinoma were imaged. Confocal images were acquired in an average of less than 2 min per core. Invasive tumor cellularity estimated from histologic and grayscale confocal images showed moderate agreement by kappa coefficient: κ = 0.48 ± 0.09 (p < 0.001). Grayscale confocal images require less than 2 min for acquisition and allow for evaluation of invasive tumor cellularity in breast core needle biopsy specimens with moderate agreement to histologic images. We show that confocal fluorescence microscopy can be performed immediately following specimen acquisition and could indicate the need for additional biopsies at the initial visit.en_US
dc.identifier.citationDobbs, Jessica, Krishnamurthy, Savitri, Kyrish, Matthew, et al.. "Confocal fluorescence microscopy for rapid evaluation of invasive tumor cellularity of inflammatory breast carcinoma core needle biopsies." <i>Breast Cancer Research and Treatment,</i> (2014) Springer: http://dx.doi.org/10.1007/s10549-014-3182-5.en_US
dc.identifier.doihttp://dx.doi.org/10.1007/s10549-014-3182-5en_US
dc.identifier.urihttps://hdl.handle.net/1911/78536en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/en_US
dc.subject.keywordinflammatory breast canceren_US
dc.subject.keywordcore needle biopsyen_US
dc.subject.keywordconfocal fluorescence microscopyen_US
dc.subject.keywordtissue samplingen_US
dc.titleConfocal fluorescence microscopy for rapid evaluation of invasive tumor cellularity of inflammatory breast carcinoma core needle biopsiesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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