In situ measurement of bovine serum albumin interaction with gold nanospheres

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dc.citation.firstpage9131
dc.citation.issueNumber24
dc.citation.journalTitleLangmuir
dc.citation.lastpage9139
dc.citation.volumeNumber28
dc.contributor.authorDominguez-Medina, Sergio
dc.contributor.authorMcDonough, Steven
dc.contributor.authorSwanglap, Pattanawit
dc.contributor.authorLandes, Christy F.
dc.contributor.authorLink, Stephan
dc.contributor.orgLaboratory for Nanophotonics
dc.date.accessioned2013-08-20T21:52:45Z
dc.date.available2013-08-20T21:52:45Z
dc.date.issued2012
dc.description.abstractHere we present in situ observations of adsorption of bovine serum albumin (BSA) on citratestabilized gold nanospheres. We implemented scattering correlation spectroscopy as a tool to quantify changes in the nanoparticle Brownian motion resulting from BSA adsorption onto the nanoparticle surface. Protein binding was observed as an increase in the nanoparticle hydrodynamic radius. Our results indicate the formation of a protein monolayer at similar albumin concentrations as those found in human blood. Additionally, by monitoring the frequency and intensity of individual scattering events caused by single gold nanoparticles passing the observation volume, we found that BSA did not induce colloidal aggregation, a relevant result from the toxicological viewpoint. Moreover, to elucidate the thermodynamics of the gold nanoparticle-BSA association, we measured an adsorption isotherm which was best described by an anti-cooperative binding model. The number of binding sites based on this model was consistent with a BSA monolayer in its native state. In contrast, experiments using poly-ethylene glycol capped gold nanoparticles revealed no evidence for adsorption of BSA.
dc.embargo.termsnone
dc.identifier.citationDominguez-Medina, Sergio, McDonough, Steven, Swanglap, Pattanawit, et al.. "In situ measurement of bovine serum albumin interaction with gold nanospheres." <i>Langmuir,</i> 28, no. 24 (2012) American Chemical Society: 9131-9139. http://dx.doi.org/10.1021/la3005213.
dc.identifier.doihttp://dx.doi.org/10.1021/la3005213
dc.identifier.urihttps://hdl.handle.net/1911/71743
dc.language.isoeng
dc.publisherAmerican Chemical Society
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.
dc.subject.keywordGold nanoparticles
dc.subject.keywordsurface plasmon
dc.subject.keywordbovine serum albumin
dc.subject.keywordprotein corona
dc.subject.keywordcorrelation spectroscopy
dc.subject.keyworddiffusion
dc.titleIn situ measurement of bovine serum albumin interaction with gold nanospheres
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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