In situ measurement of bovine serum albumin interaction with gold nanospheres

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dc.citation.firstpage9131en_US
dc.citation.issueNumber24en_US
dc.citation.journalTitleLangmuiren_US
dc.citation.lastpage9139en_US
dc.citation.volumeNumber28en_US
dc.contributor.authorDominguez-Medina, Sergioen_US
dc.contributor.authorMcDonough, Stevenen_US
dc.contributor.authorSwanglap, Pattanawiten_US
dc.contributor.authorLandes, Christy F.en_US
dc.contributor.authorLink, Stephanen_US
dc.contributor.orgLaboratory for Nanophotonicsen_US
dc.date.accessioned2013-08-20T21:52:45Zen_US
dc.date.available2013-08-20T21:52:45Zen_US
dc.date.issued2012en_US
dc.description.abstractHere we present in situ observations of adsorption of bovine serum albumin (BSA) on citratestabilized gold nanospheres. We implemented scattering correlation spectroscopy as a tool to quantify changes in the nanoparticle Brownian motion resulting from BSA adsorption onto the nanoparticle surface. Protein binding was observed as an increase in the nanoparticle hydrodynamic radius. Our results indicate the formation of a protein monolayer at similar albumin concentrations as those found in human blood. Additionally, by monitoring the frequency and intensity of individual scattering events caused by single gold nanoparticles passing the observation volume, we found that BSA did not induce colloidal aggregation, a relevant result from the toxicological viewpoint. Moreover, to elucidate the thermodynamics of the gold nanoparticle-BSA association, we measured an adsorption isotherm which was best described by an anti-cooperative binding model. The number of binding sites based on this model was consistent with a BSA monolayer in its native state. In contrast, experiments using poly-ethylene glycol capped gold nanoparticles revealed no evidence for adsorption of BSA.en_US
dc.embargo.termsnoneen_US
dc.identifier.citationDominguez-Medina, Sergio, McDonough, Steven, Swanglap, Pattanawit, et al.. "In situ measurement of bovine serum albumin interaction with gold nanospheres." <i>Langmuir,</i> 28, no. 24 (2012) American Chemical Society: 9131-9139. http://dx.doi.org/10.1021/la3005213.en_US
dc.identifier.doihttp://dx.doi.org/10.1021/la3005213en_US
dc.identifier.urihttps://hdl.handle.net/1911/71743en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.en_US
dc.subject.keywordGold nanoparticlesen_US
dc.subject.keywordsurface plasmonen_US
dc.subject.keywordbovine serum albuminen_US
dc.subject.keywordprotein coronaen_US
dc.subject.keywordcorrelation spectroscopyen_US
dc.subject.keyworddiffusionen_US
dc.titleIn situ measurement of bovine serum albumin interaction with gold nanospheresen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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