Aptamer-Targeted Gold Nanoparticles As Molecular-Specific Contrast Agents for Reflectance Imaging

Simple item page
dc.citation.firstpage1309en_US
dc.citation.issueNumber6en_US
dc.citation.journalTitleBioconjugate Chemistryen_US
dc.citation.lastpage1312en_US
dc.citation.volumeNumber19en_US
dc.contributor.authorJavier, David J.en_US
dc.contributor.authorNitin, Nitinen_US
dc.contributor.authorLevy, Matthewen_US
dc.contributor.authorEllington, Andrewen_US
dc.contributor.authorRichards-Kortum, Rebeccaen_US
dc.date.accessioned2016-04-04T21:23:36Zen_US
dc.date.available2016-04-04T21:23:36Zen_US
dc.date.issued2008en_US
dc.description.abstractTargeted metallic nanoparticles have shown potential as a platform for development of molecular-specific contrast agents. Aptamers have recently been demonstrated as ideal candidates for molecular targeting applications. In this study, we investigated the development of aptamer-based gold nanoparticles as contrast agents, using aptamers as targeting agents and gold nanoparticles as imaging agents. We devised a novel conjugation approach using an extended aptamer design where the extension is complementary to an oligonucleotide sequence attached to the surface of the gold nanoparticles. The chemical and optical properties of the aptamer−gold conjugates were characterized using size measurements and oligonucleotide quantitation assays. We demonstrate this conjugation approach to create a contrast agent designed for detection of prostate-specific membrane antigen (PSMA), obtaining reflectance images of PSMA(+) and PSMA(−) cell lines treated with the anti-PSMA aptamer−gold conjugates. This design strategy can easily be modified to incorporate multifunctional agents as part of a multimodal platform for reflectance imaging applications.en_US
dc.identifier.citationJavier, David J., Nitin, Nitin, Levy, Matthew, et al.. "Aptamer-Targeted Gold Nanoparticles As Molecular-Specific Contrast Agents for Reflectance Imaging." <i>Bioconjugate Chemistry,</i> 19, no. 6 (2008) American Chemical Society: 1309-1312. http://dx.doi.org/10.1021/bc8001248.en_US
dc.identifier.doihttp://dx.doi.org/10.1021/bc8001248en_US
dc.identifier.urihttps://hdl.handle.net/1911/88848en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis is an open access article published under an ACS AuthorChoiceﾠLicense, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.en_US
dc.rights.urihttp://pubs.acs.org/page/policy/authorchoice_termsofuse.htmlen_US
dc.titleAptamer-Targeted Gold Nanoparticles As Molecular-Specific Contrast Agents for Reflectance Imagingen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
bc8001248.pdf
Size:
133.93 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Faculty Publications
Bioengineering Publications