A portable regulatory RNA array design enables tunable and complex regulation across diverse bacteria

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dc.citation.articleNumber5268en_US
dc.citation.journalTitleNature Communicationsen_US
dc.citation.volumeNumber14en_US
dc.contributor.authorLiu, Baiyangen_US
dc.contributor.authorSamaniego, Christian Cubaen_US
dc.contributor.authorBennett, Matthew R.en_US
dc.contributor.authorFranco, Elisaen_US
dc.contributor.authorChappell, Jamesen_US
dc.contributor.orgBioengineeringen_US
dc.contributor.orgBiosciencesen_US
dc.date.accessioned2024-05-03T15:51:17Zen_US
dc.date.available2024-05-03T15:51:17Zen_US
dc.date.issued2023en_US
dc.description.abstractA lack of composable and tunable gene regulators has hindered efforts to engineer non-model bacteria and consortia. Toward addressing this, we explore the broad-host potential of small transcription activating RNA (STAR) and propose a design strategy to achieve tunable gene control. First, we demonstrate that STARs optimized for E. coli function across different Gram-negative species and can actuate using phage RNA polymerase, suggesting that RNA systems acting at the level of transcription are portable. Second, we explore an RNA design strategy that uses arrays of tandem and transcriptionally fused RNA regulators to precisely alter regulator concentration from 1 to 8 copies. This provides a simple means to predictably tune output gain across species and does not require access to large regulatory part libraries. Finally, we show RNA arrays can be used to achieve tunable cascading and multiplexing circuits across species, analogous to the motifs used in artificial neural networks.en_US
dc.identifier.citationLiu, B., Samaniego, C. C., Bennett, M. R., Franco, E., & Chappell, J. (2023). A portable regulatory RNA array design enables tunable and complex regulation across diverse bacteria. Nature Communications, 14(1), 5268. https://doi.org/10.1038/s41467-023-40785-xen_US
dc.identifier.digitals41467-023-40785-xen_US
dc.identifier.doihttps://doi.org/10.1038/s41467-023-40785-xen_US
dc.identifier.urihttps://hdl.handle.net/1911/115606en_US
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.rightsExcept where otherwise noted, this work is licensed under a Creative Commons Attribution (CC BY) license. Permission to reuse, publish, or reproduce the work beyond the terms of the license or beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleA portable regulatory RNA array design enables tunable and complex regulation across diverse bacteriaen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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