Ultraspecific and Highly Sensitive Nucleic Acid Detection by Integrating a DNA Catalytic Network with a Label-Free Microcavity

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dc.citation.firstpage2067
dc.citation.issueNumber10
dc.citation.journalTitleSmall
dc.citation.lastpage2076
dc.citation.volumeNumber10
dc.contributor.authorWu, Yuqiang
dc.contributor.authorZhang, David Yu
dc.contributor.authorYin, Peng
dc.contributor.authorVollmer, Frank
dc.date.accessioned2015-07-10T18:42:19Z
dc.date.available2015-07-10T18:42:19Z
dc.date.issued2014
dc.description.abstractNucleic acid detection with label-free biosensors circumvents costly fluorophore functionalization steps associated with conventional assays by utilizing transducers of impressive ultimate detection limits. Despite this technological prowess, molecular recognition at a surface limits the biosensors' sensitivity, specificity, and reusability. It is therefore imperative to integrate novel molecular approaches with existing label-free transducers to overcome those limitations. Here, we demonstrate this concept by integrating a DNA strand displacement circuit with a micron-scale whispering gallery mode (WGM) microsphere biosensor. The integrated biosensor exhibits at least 25-fold improved nucleic acid sensitivity, and sets a new record for label-free microcavity biosensors by detecting 80 pM (32 fmol) of a 22nt oligomer; this improvement results from the catalytic behavior of the circuit. Furthermore, the integrated sensor exhibits extremely high specificity; single nucleotide variants yield 40- to 100-fold lower signal. Finally, the same physical sensor was demonstrated to alternatingly detect 2 different nucleic acid sequences through 5 cycles of detection, showcasing both its reusability and its versatility.
dc.identifier.citationWu, Yuqiang, Zhang, David Yu, Yin, Peng, et al.. "Ultraspecific and Highly Sensitive Nucleic Acid Detection by Integrating a DNA Catalytic Network with a Label-Free Microcavity." <i>Small,</i> 10, no. 10 (2014) Wiley: 2067-2076. http://dx.doi.org/10.1002/smll.201303558.
dc.identifier.doihttp://dx.doi.org/10.1002/smll.201303558
dc.identifier.urihttps://hdl.handle.net/1911/80891
dc.language.isoeng
dc.publisherWiley
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.
dc.subject.keywordbiosensor
dc.subject.keywordDNA catalytic circuit
dc.subject.keywordoligonucleotides
dc.subject.keywordoptical microcavity
dc.subject.keywordsingle-nucleotide polymorphism detection
dc.titleUltraspecific and Highly Sensitive Nucleic Acid Detection by Integrating a DNA Catalytic Network with a Label-Free Microcavity
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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