Repair of complex ovine segmental mandibulectomy utilizing customized tissue engineered bony flaps

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dc.citation.articleNumbere0280481en_US
dc.citation.issueNumber2en_US
dc.citation.journalTitlePLOS ONEen_US
dc.citation.volumeNumber18en_US
dc.contributor.authorWatson, Emmaen_US
dc.contributor.authorPearce, Hannah A.en_US
dc.contributor.authorHogan, Katie J.en_US
dc.contributor.authorDijk, Natasja W.M. vanen_US
dc.contributor.authorSmoak, Mollie M.en_US
dc.contributor.authorBarrios, Sergioen_US
dc.contributor.authorSmith, Brandon T.en_US
dc.contributor.authorTatara, Alexander M.en_US
dc.contributor.authorWoernley, Timothy C.en_US
dc.contributor.authorShum, Jonathanen_US
dc.contributor.authorPearl, Craig B.en_US
dc.contributor.authorMelville, James C.en_US
dc.contributor.authorHo, Tangen_US
dc.contributor.authorHanna, Issa A.en_US
dc.contributor.authorDemian, Nagien_US
dc.contributor.authorBeucken, Jeroen J.J.P. van denen_US
dc.contributor.authorJansen, John A.en_US
dc.contributor.authorWong, Mark E.en_US
dc.contributor.authorMikos, Antonios G.en_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2023-03-10T19:04:15Zen_US
dc.date.available2023-03-10T19:04:15Zen_US
dc.date.issued2023en_US
dc.description.abstractCraniofacial defects require a treatment approach that provides both robust tissues to withstand the forces of mastication and high geometric fidelity that allows restoration of facial architecture. When the surrounding soft tissue is compromised either through lack of quantity (insufficient soft tissue to enclose a graft) or quality (insufficient vascularity or inducible cells), a vascularized construct is needed for reconstruction. Tissue engineering using customized 3D printed bioreactors enables the generation of mechanically robust, vascularized bony tissues of the desired geometry. While this approach has been shown to be effective when utilized for reconstruction of non-load bearing ovine angular defects and partial segmental defects, the two-stage approach to mandibular reconstruction requires testing in a large, load-bearing defect. In this study, 5 sheep underwent bioreactor implantation and the creation of a load-bearing mandibular defect. Two bioreactor geometries were tested: a larger complex bioreactor with a central groove, and a smaller rectangular bioreactor that were filled with a mix of xenograft and autograft (initial bone volume/total volume BV/TV of 31.8 ± 1.6%). At transfer, the tissues generated within large and small bioreactors were composed of a mix of lamellar and woven bone and had BV/TV of 55.3 ± 2.6% and 59.2 ± 6.3%, respectively. After transfer of the large bioreactors to the mandibular defect, the bioreactor tissues continued to remodel, reaching a final BV/TV of 64.5 ± 6.2%. Despite recalcitrant infections, viable osteoblasts were seen within the transferred tissues to the mandibular site at the end of the study, suggesting that a vascularized customized bony flap is a potentially effective reconstructive strategy when combined with an optimal stabilization strategy and local antibiotic delivery prior to development of a deep-seated infection.en_US
dc.identifier.citationWatson, Emma, Pearce, Hannah A., Hogan, Katie J., et al.. "Repair of complex ovine segmental mandibulectomy utilizing customized tissue engineered bony flaps." <i>PLOS ONE,</i> 18, no. 2 (2023) Public Library of Science: https://doi.org/10.1371/journal.pone.0280481.en_US
dc.identifier.digitaljournal-pone-0280481en_US
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0280481en_US
dc.identifier.urihttps://hdl.handle.net/1911/114504en_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleRepair of complex ovine segmental mandibulectomy utilizing customized tissue engineered bony flapsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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