Unique patterns of transcript and miRNA expression in the South American strong voltage electric eel (Electrophorus electricus)

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dc.citation.journalTitleBMC Genomicsen_US
dc.contributor.authorTraeger, Lindsay L.en_US
dc.contributor.authorVolkening, Jeremy D.en_US
dc.contributor.authorMoffett, Howellen_US
dc.contributor.authorGallant, Jason R.en_US
dc.contributor.authorChen, Po-Haoen_US
dc.contributor.authorNovina, Carl D.en_US
dc.contributor.authorPhillips, George N.Jr.en_US
dc.contributor.authorAnand, Reneen_US
dc.contributor.authorWells, Gregg B.en_US
dc.contributor.authorPinch, Matthewen_US
dc.contributor.authorGüth, Roberten_US
dc.contributor.authorUnguez, Graciela A.en_US
dc.contributor.authorAlbert, James S.en_US
dc.contributor.authorZakon, Harolden_US
dc.contributor.authorSussman, Michael R.en_US
dc.contributor.authorSamanta, Manoj P.en_US
dc.date.accessioned2015-09-16T17:51:03Zen_US
dc.date.available2015-09-16T17:51:03Zen_US
dc.date.issued2015en_US
dc.date.updated2015-09-16T17:51:03Zen_US
dc.description.abstractBackground: With its unique ability to produce high-voltage electric discharges in excess of 600 volts, the South American strong voltage electric eel (Electrophorus electricus) has played an important role in the history of science. Remarkably little is understood about the molecular nature of its electric organs. Results: We present an in-depth analysis of the genome of E. electricus, including the transcriptomes of eight mature tissues: brain, spinal cord, kidney, heart, skeletal muscle, Sachs’ electric organ, main electric organ, and Hunter’s electric organ. A gene set enrichment analysis based on gene ontology reveals enriched functions in all three electric organs related to transmembrane transport, androgen binding, and signaling. This study also represents the first analysis of miRNA in electric fish. It identified a number of miRNAs displaying electric organ-specific expression patterns, including one novel miRNA highly over-expressed in all three electric organs of E. electricus. All three electric organ tissues also express three conserved miRNAs that have been reported to inhibit muscle development in mammals, suggesting that miRNA-dependent regulation of gene expression might play an important role in specifying an electric organ identity from its muscle precursor. These miRNA data were supported using another complete miRNA profile from muscle and electric organ tissues of a second gymnotiform species. Conclusions: ur work on the E. electricus genome and eight tissue-specific gene expression profiles will greatly facilitate future research on determining the coding and regulatory sequences that specify the function, development, and evolution of electric organs. Moreover, these data and future studies will be informed by the first comprehensive analysis of miRNA expression in an electric fish presented here.en_US
dc.identifier.citationTraeger, Lindsay L., Volkening, Jeremy D., Moffett, Howell, et al.. "Unique patterns of transcript and miRNA expression in the South American strong voltage electric eel (Electrophorus electricus)." <i>BMC Genomics,</i> (2015) BioMed Central: http://dx.doi.org/10.1186/s12864-015-1288-8.en_US
dc.identifier.doihttp://dx.doi.org/10.1186/s12864-015-1288-8en_US
dc.identifier.urihttps://hdl.handle.net/1911/81649en_US
dc.language.isoengen_US
dc.publisherBioMed Centralen_US
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en_US
dc.rights.holderTraeger et al.; licensee BioMed Central.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleUnique patterns of transcript and miRNA expression in the South American strong voltage electric eel (Electrophorus electricus)en_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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