The Drosophila melanogaster attP40 docking site and derivatives are insertion mutations of msp-300

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dc.citation.articleNumbere0278598en_US
dc.citation.issueNumber12en_US
dc.citation.journalTitlePLOS ONEen_US
dc.citation.volumeNumber17en_US
dc.contributor.authorGraaf, Kevin van deren_US
dc.contributor.authorSrivastav, Saurabhen_US
dc.contributor.authorSingh, Pratibhaen_US
dc.contributor.authorMcNew, James A.en_US
dc.contributor.authorStern, Michaelen_US
dc.date.accessioned2023-01-27T14:47:28Zen_US
dc.date.available2023-01-27T14:47:28Zen_US
dc.date.issued2022en_US
dc.description.abstractThe ɸC31 integrase system is widely used in Drosophila melanogaster to allow transgene targeting to specific loci. Over the years, flies bearing any of more than 100 attP docking sites have been constructed. One popular docking site, termed attP40, is located close to the Nesprin-1 orthologue msp-300 and lies upstream of certain msp-300 isoforms and within the first intron of others. Here we show that attP40 causes larval muscle nuclear clustering, which is a phenotype also conferred by msp-300 mutations. We also show that flies bearing insertions within attP40 can exhibit decreased msp-300 transcript levels in third instar larvae. Finally, chromosomes carrying certain “transgenic RNAi project” (TRiP) insertions into attP40 can confer pupal or adult inviability or infertility, or dominant nuclear clustering effects in certain genetic backgrounds. These phenotypes do not require transcription from the insertions within attP40. These results demonstrate that attP40 and insertion derivatives act as msp-300 insertional mutations. These findings should be considered when interpreting data from attP40-bearing flies.en_US
dc.identifier.citationGraaf, Kevin van der, Srivastav, Saurabh, Singh, Pratibha, et al.. "The Drosophila melanogaster attP40 docking site and derivatives are insertion mutations of msp-300." <i>PLOS ONE,</i> 17, no. 12 (2022) Public Library of Science: https://doi.org/10.1371/journal.pone.0278598.en_US
dc.identifier.digitaljournal-pone-0278598en_US
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0278598en_US
dc.identifier.urihttps://hdl.handle.net/1911/114279en_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleThe Drosophila melanogaster attP40 docking site and derivatives are insertion mutations of msp-300en_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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