In vitroﾠandﾠin vivoﾠevaluation of self-mineralization and biocompatibility of injectable, dual-gelling hydrogels for bone tissue engineering

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dc.citation.firstpage25en_US
dc.citation.journalTitleJournal of Controlled Releaseen_US
dc.citation.lastpage34en_US
dc.citation.volumeNumber205en_US
dc.contributor.authorVo, Tiffany N.en_US
dc.contributor.authorEkenseair, Adam K.en_US
dc.contributor.authorSpicer, Patrick P.en_US
dc.contributor.authorWatson, Brendan M.en_US
dc.contributor.authorTzouanas, Stephanie N.en_US
dc.contributor.authorRoh, Terrence T.en_US
dc.contributor.authorMikos, Antonios G.en_US
dc.date.accessioned2016-08-30T20:50:15Zen_US
dc.date.available2016-08-30T20:50:15Zen_US
dc.date.issued2015en_US
dc.description.abstractIn this study, we investigated the mineralization capacity and biocompatibility of injectable, dual-gelling hydrogels in a rat cranial defect as a function of hydrogel hydrophobicity from either the copolymerization of a hydrolyzable lactone ring or the hydrogel polymer content. The hydrogel system comprised a poly(N-isopropylacrylamide)-based thermogelling macromer (TGM) and a polyamidoamine crosslinker. The thermogelling macromer was copolymerized with (TGM/DBA) or without (TGM) a dimethyl-γ-butyrolactone acrylate (DBA)-containing lactone ring that modulated the lower critical solution temperature and thus, the hydrogel hydrophobicity, over time. Three hydrogel groups were examined: (1) 15wt.% TGM, (2) 15wt.% TGM/DBA, and (3) 20wt.% TGM/DBA. The hydrogels were implanted within an 8mm critical size rat cranial defect for 4 and 12weeks. Implants were harvested at each timepoint and analyzed for bone formation, hydrogel mineralization and tissue response using microcomputed tomography (microCT). Histology and fibrous capsule scoring showed a light inflammatory response at 4weeks that was mitigated by 12weeks for all groups. MicroCT scoring and bone volume quantification demonstrated a similar bone formation at 4weeks that was significantly increased for the more hydrophobic hydrogel formulations - 15wt.% TGM and 20wt.% TGM/DBA - from 4weeks to 12weeks. A complementary in vitro acellular mineralization study revealed that the hydrogels exhibited calcium binding properties in the presence of serum-containing media, which was modulated by the hydrogel hydrophobicity. The tailored mineralization capacity of these injectable, dual-gelling hydrogels with hydrolysis-dependent hydrophobicity presents an exciting property for their use in bone tissue engineering applications.en_US
dc.identifier.citationVo, Tiffany N., Ekenseair, Adam K., Spicer, Patrick P., et al.. "In vitroﾠandﾠin vivoﾠevaluation of self-mineralization and biocompatibility of injectable, dual-gelling hydrogels for bone tissue engineering." <i>Journal of Controlled Release,</i> 205, (2015) Elsevier: 25-34. http://dx.doi.org/10.1016/j.jconrel.2014.11.028.en_US
dc.identifier.doihttp://dx.doi.org/10.1016/j.jconrel.2014.11.028en_US
dc.identifier.urihttps://hdl.handle.net/1911/91365en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.en_US
dc.subject.keywordboneen_US
dc.subject.keywordPoly(N-isopropylacrylamide)en_US
dc.subject.keywordthermogellingen_US
dc.subject.keywordtissue engineeringen_US
dc.titleIn vitroﾠandﾠin vivoﾠevaluation of self-mineralization and biocompatibility of injectable, dual-gelling hydrogels for bone tissue engineeringen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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