Proteomic analyses reveal distinct chromatin‐associated and soluble transcription factor complexes

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dc.citation.journalTitleMolecular Systems Biologyen_US
dc.citation.volumeNumber11en_US
dc.contributor.authorLi, Xuen_US
dc.contributor.authorWang, Wenqien_US
dc.contributor.authorWang, Jiadongen_US
dc.contributor.authorMalovannaya, Annaen_US
dc.contributor.authorXi, Yuanxinen_US
dc.contributor.authorLi, Weien_US
dc.contributor.authorGuerra, Rudyen_US
dc.contributor.authorHawke, David H.en_US
dc.contributor.authorQin, Junen_US
dc.contributor.authorChen, Junjieen_US
dc.date.accessioned2015-03-02T16:58:32Zen_US
dc.date.available2015-03-02T16:58:32Zen_US
dc.date.issued2015en_US
dc.description.abstractThe current knowledge on how transcription factors (TFs), the ultimate targets and executors of cellular signalling pathways, are regulated by protein–protein interactions remains limited. Here, we performed proteomics analyses of soluble and chromatin‐associated complexes of 56 TFs, including the targets of many signalling pathways involved in development and cancer, and 37 members of the Forkhead box (FOX) TF family. Using tandem affinity purification followed by mass spectrometry (TAP/MS), we performed 214 purifications and identified 2,156 high‐confident protein–protein interactions. We found that most TFs form very distinct protein complexes on and off chromatin. Using this data set, we categorized the transcription‐related or unrelated regulators for general or specific TFs. Our study offers a valuable resource of protein–protein interaction networks for a large number of TFs and underscores the general principle that TFs form distinct location‐specific protein complexes that are associated with the different regulation and diverse functions of these Tfs.en_US
dc.identifier.citationLi, Xu, Wang, Wenqi, Wang, Jiadong, et al.. "Proteomic analyses reveal distinct chromatin‐associated and soluble transcription factor complexes." <i>Molecular Systems Biology,</i> 11, (2015) EMBO: http://dx.doi.org/10.15252/msb.20145504.en_US
dc.identifier.doihttp://dx.doi.org/10.15252/msb.20145504en_US
dc.identifier.urihttps://hdl.handle.net/1911/79056en_US
dc.language.isoengen_US
dc.publisherEMBOen_US
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordorkhead boxen_US
dc.subject.keywordmass spectrometryen_US
dc.subject.keywordprotein-protein interactionen_US
dc.subject.keywordtranscriptional factoren_US
dc.titleProteomic analyses reveal distinct chromatin‐associated and soluble transcription factor complexesen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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