Optogenetic control of gut bacterial metabolism to promote longevity

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dc.citation.articleNumbere56849en_US
dc.citation.journalTitleeLifeen_US
dc.citation.volumeNumber9en_US
dc.contributor.authorHartsough, Lucas A.en_US
dc.contributor.authorPark, Mooncheolen_US
dc.contributor.authorKotlajich, Matthew V.en_US
dc.contributor.authorLazar, John Tyleren_US
dc.contributor.authorHan, Bingen_US
dc.contributor.authorLin, Chih-Chun J.en_US
dc.contributor.authorMusteata, Elenaen_US
dc.contributor.authorGambill, Laurenen_US
dc.contributor.authorWang, Meng C.en_US
dc.contributor.authorTabor, Jeffrey J.en_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2021-02-08T18:37:52Zen_US
dc.date.available2021-02-08T18:37:52Zen_US
dc.date.issued2020en_US
dc.description.abstractGut microbial metabolism is associated with host longevity. However, because it requires direct manipulation of microbial metabolism in situ, establishing a causal link between these two processes remains challenging. We demonstrate an optogenetic method to control gene expression and metabolite production from bacteria residing in the host gut. We genetically engineer an Escherichia coli strain that secretes colanic acid (CA) under the quantitative control of light. Using this optogenetically-controlled strain to induce CA production directly in the Caenorhabditis elegans gut, we reveal the local effect of CA in protecting intestinal mitochondria from stress-induced hyper-fragmentation. We also demonstrate that the lifespan-extending effect of this strain is positively correlated with the intensity of green light, indicating a dose-dependent CA benefit on the host. Thus, optogenetics can be used to achieve quantitative and temporal control of gut bacterial metabolism in order to reveal its local and systemic effects on host health and aging.en_US
dc.identifier.citationHartsough, Lucas A., Park, Mooncheol, Kotlajich, Matthew V., et al.. "Optogenetic control of gut bacterial metabolism to promote longevity." <i>eLife,</i> 9, (2020) eLife Sciences Publications, Ltd: https://doi.org/10.7554/eLife.56849.en_US
dc.identifier.digitalelife-56849-v1en_US
dc.identifier.doihttps://doi.org/10.7554/eLife.56849en_US
dc.identifier.urihttps://hdl.handle.net/1911/109816en_US
dc.language.isoengen_US
dc.publishereLife Sciences Publications, Ltden_US
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleOptogenetic control of gut bacterial metabolism to promote longevityen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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