A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping

Simple item page
dc.citation.articleNumber28en_US
dc.citation.journalTitleBMC Biologyen_US
dc.citation.volumeNumber20en_US
dc.contributor.authorLusk, Savannah J.en_US
dc.contributor.authorMcKinney, Andrewen_US
dc.contributor.authorHunt, Patrick J.en_US
dc.contributor.authorFahey, Paul G.en_US
dc.contributor.authorPatel, Jayen_US
dc.contributor.authorChang, Andersenen_US
dc.contributor.authorSun, Jenny J.en_US
dc.contributor.authorMartinez, Vena K.en_US
dc.contributor.authorZhu, Ping Junen_US
dc.contributor.authorEgbert, Jeremy R.en_US
dc.contributor.authorAllen, Geneveraen_US
dc.contributor.authorJiang, Xiaolongen_US
dc.contributor.authorArenkiel, Benjamin R.en_US
dc.contributor.authorTolias, Andreas S.en_US
dc.contributor.authorCosta-Mattioli, Mauroen_US
dc.contributor.authorRay, Russell S.en_US
dc.date.accessioned2022-03-24T13:31:47Zen_US
dc.date.available2022-03-24T13:31:47Zen_US
dc.date.issued2022en_US
dc.description.abstractThe functional understanding of genetic interaction networks and cellular mechanisms governing health and disease requires the dissection, and multifaceted study, of discrete cell subtypes in developing and adult animal models. Recombinase-driven expression of transgenic effector alleles represents a significant and powerful approach to delineate cell populations for functional, molecular, and anatomical studies. In addition to single recombinase systems, the expression of two recombinases in distinct, but partially overlapping, populations allows for more defined target expression. Although the application of this method is becoming increasingly popular, its experimental implementation has been broadly restricted to manipulations of a limited set of common alleles that are often commercially produced at great expense, with costs and technical challenges associated with production of intersectional mouse lines hindering customized approaches to many researchers. Here, we present a simplified CRISPR toolkit for rapid, inexpensive, and facile intersectional allele production.en_US
dc.identifier.citationLusk, Savannah J., McKinney, Andrew, Hunt, Patrick J., et al.. "A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping." <i>BMC Biology,</i> 20, (2022) Springer Nature: https://doi.org/10.1186/s12915-022-01227-0.en_US
dc.identifier.digitals12915-022-01227-0en_US
dc.identifier.doihttps://doi.org/10.1186/s12915-022-01227-0en_US
dc.identifier.urihttps://hdl.handle.net/1911/112058en_US
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/.en_US
dc.titleA CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mappingen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s12915-022-01227-0.pdf
Size:
9.86 MB
Format:
Adobe Portable Document Format
Download
Collections
Faculty Publications
Computer Science Publications
ECE Publications
Statistics Publications