The sound of silence: Transgene silencing in mammalian cell engineering

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dc.citation.firstpage950en_US
dc.citation.issueNumber12en_US
dc.citation.journalTitleCell Systemsen_US
dc.citation.lastpage973en_US
dc.citation.volumeNumber13en_US
dc.contributor.authorCabrera, Alanen_US
dc.contributor.authorEdelstein, Hailey I.en_US
dc.contributor.authorGlykofrydis, Fokionen_US
dc.contributor.authorLove, Kasey S.en_US
dc.contributor.authorPalacios, Sebastianen_US
dc.contributor.authorTycko, Joshen_US
dc.contributor.authorZhang, Mengen_US
dc.contributor.authorLensch, Sarahen_US
dc.contributor.authorShields, Cara E.en_US
dc.contributor.authorLivingston, Marken_US
dc.contributor.authorWeiss, Ronen_US
dc.contributor.authorZhao, Huiminen_US
dc.contributor.authorHaynes, Karmella A.en_US
dc.contributor.authorMorsut, Leonardoen_US
dc.contributor.authorChen, Yvonne Y.en_US
dc.contributor.authorKhalil, Ahmad S.en_US
dc.contributor.authorWong, Wilson W.en_US
dc.contributor.authorCollins, James J.en_US
dc.contributor.authorRosser, Susan J.en_US
dc.contributor.authorPolizzi, Karenen_US
dc.contributor.authorElowitz, Michael B.en_US
dc.contributor.authorFussenegger, Martinen_US
dc.contributor.authorHilton, Isaac B.en_US
dc.contributor.authorLeonard, Joshua N.en_US
dc.contributor.authorBintu, Lacramioaraen_US
dc.contributor.authorGalloway, Kate E.en_US
dc.contributor.authorDeans, Tara L.en_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2023-01-27T14:47:07Zen_US
dc.date.available2023-01-27T14:47:07Zen_US
dc.date.issued2022en_US
dc.description.abstractTo elucidate principles operating in native biological systems and to develop novel biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within native transcriptional networks. The utility of synthetic gene circuits for cell engineering relies on the ability to control the expression of all constituent transgene components. Transgene silencing, defined as the loss of expression over time, persists as an obstacle for engineering primary cells and stem cells with transgenic cargos. In this review, we highlight the challenge that transgene silencing poses to the robust engineering of mammalian cells, outline potential molecular mechanisms of silencing, and present approaches for preventing transgene silencing. We conclude with a perspective identifying future research directions for improving the performance of synthetic gene circuits.en_US
dc.identifier.citationCabrera, Alan, Edelstein, Hailey I., Glykofrydis, Fokion, et al.. "The sound of silence: Transgene silencing in mammalian cell engineering." <i>Cell Systems,</i> 13, no. 12 (2022) Cell Press: 950-973. https://doi.org/10.1016/j.cels.2022.11.005.en_US
dc.identifier.digital1-s2-0-S2405471222004641-mainen_US
dc.identifier.doihttps://doi.org/10.1016/j.cels.2022.11.005en_US
dc.identifier.urihttps://hdl.handle.net/1911/114246en_US
dc.language.isoengen_US
dc.publisherCell Pressen_US
dc.rightsThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/).en_US
dc.titleThe sound of silence: Transgene silencing in mammalian cell engineeringen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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