The sound of silence: Transgene silencing in mammalian cell engineering

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dc.citation.firstpage950
dc.citation.issueNumber12
dc.citation.journalTitleCell Systems
dc.citation.lastpage973
dc.citation.volumeNumber13
dc.contributor.authorCabrera, Alan
dc.contributor.authorEdelstein, Hailey I.
dc.contributor.authorGlykofrydis, Fokion
dc.contributor.authorLove, Kasey S.
dc.contributor.authorPalacios, Sebastian
dc.contributor.authorTycko, Josh
dc.contributor.authorZhang, Meng
dc.contributor.authorLensch, Sarah
dc.contributor.authorShields, Cara E.
dc.contributor.authorLivingston, Mark
dc.contributor.authorWeiss, Ron
dc.contributor.authorZhao, Huimin
dc.contributor.authorHaynes, Karmella A.
dc.contributor.authorMorsut, Leonardo
dc.contributor.authorChen, Yvonne Y.
dc.contributor.authorKhalil, Ahmad S.
dc.contributor.authorWong, Wilson W.
dc.contributor.authorCollins, James J.
dc.contributor.authorRosser, Susan J.
dc.contributor.authorPolizzi, Karen
dc.contributor.authorElowitz, Michael B.
dc.contributor.authorFussenegger, Martin
dc.contributor.authorHilton, Isaac B.
dc.contributor.authorLeonard, Joshua N.
dc.contributor.authorBintu, Lacramioara
dc.contributor.authorGalloway, Kate E.
dc.contributor.authorDeans, Tara L.
dc.date.accessioned2023-01-27T14:47:07Z
dc.date.available2023-01-27T14:47:07Z
dc.date.issued2022
dc.description.abstractTo elucidate principles operating in native biological systems and to develop novel biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within native transcriptional networks. The utility of synthetic gene circuits for cell engineering relies on the ability to control the expression of all constituent transgene components. Transgene silencing, defined as the loss of expression over time, persists as an obstacle for engineering primary cells and stem cells with transgenic cargos. In this review, we highlight the challenge that transgene silencing poses to the robust engineering of mammalian cells, outline potential molecular mechanisms of silencing, and present approaches for preventing transgene silencing. We conclude with a perspective identifying future research directions for improving the performance of synthetic gene circuits.
dc.identifier.citationCabrera, Alan, Edelstein, Hailey I., Glykofrydis, Fokion, et al.. "The sound of silence: Transgene silencing in mammalian cell engineering." <i>Cell Systems,</i> 13, no. 12 (2022) Cell Press: 950-973. https://doi.org/10.1016/j.cels.2022.11.005.
dc.identifier.digital1-s2-0-S2405471222004641-main
dc.identifier.doihttps://doi.org/10.1016/j.cels.2022.11.005
dc.identifier.urihttps://hdl.handle.net/1911/114246
dc.language.isoeng
dc.publisherCell Press
dc.rightsThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.titleThe sound of silence: Transgene silencing in mammalian cell engineering
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpublisher version
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