Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation

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dc.citation.firstpagee0130749en_US
dc.citation.issueNumber6en_US
dc.citation.journalTitlePLoS ONEen_US
dc.citation.volumeNumber10en_US
dc.contributor.authorBalaoing, Liezl Raeen_US
dc.contributor.authorPost, Allison Davisen_US
dc.contributor.authorLin, Adam Yuhen_US
dc.contributor.authorTseng, Huberten_US
dc.contributor.authorMoake, Joel L.en_US
dc.contributor.authorGrande-Allen, K. Janeen_US
dc.date.accessioned2016-04-01T20:24:37Zen_US
dc.date.available2016-04-01T20:24:37Zen_US
dc.date.issued2015en_US
dc.description.abstractValve endothelial cells (VEC) have unique phenotypic responses relative to other types of vascular endothelial cells and have highly sensitive hemostatic functions affected by changes in valve tissues. Furthermore, effects of environmental factors on VEC hemostatic function has not been characterized. This work used a poly(ethylene glycol) diacrylate (PEGDA) hydrogel platform to evaluate the effects of substrate stiffness and cell adhesive ligands on VEC phenotype and expression of hemostatic genes. Hydrogels of molecular weights (MWs) 3.4, 8, and 20 kDa were polymerized into platforms of different rigidities and thiol-modified cell adhesive peptides were covalently bound to acrylate groups on the hydrogel surfaces. The peptide RKRLQVQLSIRT (RKR) is a syndecan-1 binding ligand derived from laminin, a trimeric protein and a basement membrane matrix component. Conversely, RGDS is an integrin binding peptide found in many extracellular matrix (ECM) proteins including fibronectin, fibrinogen, and von Willebrand factor (VWF). VECs adhered to and formed a stable monolayer on all RKR-coated hydrogel-MW combinations. RGDS-coated platforms supported VEC adhesion and growth on RGDS-3.4 kDa and RGDS-8 kDa hydrogels. VECs cultured on the softer RKR-8 kDa and RKR-20 kDa hydrogel platforms had significantly higher gene expression for all anti-thrombotic (ADAMTS-13, tissue factor pathway inhibitor, and tissue plasminogen activator) and thrombotic (VWF, tissue factor, and P-selectin) proteins than VECs cultured on RGDS-coated hydrogels and tissue culture polystyrene controls. Stimulated VECs promoted greater platelet adhesion than non-stimulated VECs on their respective culture condition; yet stimulated VECs on RGDS-3.4 kDa gels were not as responsive to stimulation relative to the RKR-gel groups. Thus, the syndecan binding, laminin-derived peptide promoted stable VEC adhesion on the softer hydrogels and maintained VEC phenotype and natural hemostatic function. In conclusion, utilization of non-integrin adhesive peptide sequences derived from basement membrane ECM may recapitulate balanced VEC function and may benefit endothelialization of valve implants.en_US
dc.identifier.citationBalaoing, Liezl Rae, Post, Allison Davis, Lin, Adam Yuh, et al.. "Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation." <i>PLoS ONE,</i> 10, no. 6 (2015) Public Library of Science: e0130749. http://dx.doi.org/10.1371/journal.pone.0130749.en_US
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0130749en_US
dc.identifier.urihttps://hdl.handle.net/1911/88820en_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.titleLaminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulationen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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