Hyaluronan Hydrogels for a Biomimetic Spongiosa Layer of Tissue Engineered Heart Valve Scaffolds

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dc.citation.firstpage1766en_US
dc.citation.issueNumber5en_US
dc.citation.journalTitleBiomacromoleculesen_US
dc.citation.lastpage1775en_US
dc.citation.volumeNumber17en_US
dc.contributor.authorPuperi, Daniel S.en_US
dc.contributor.authorO’Connell, Ronan W.en_US
dc.contributor.authorPunske, Zoe E.en_US
dc.contributor.authorWu, Yanen_US
dc.contributor.authorWest, Jennifer L.en_US
dc.contributor.authorGrande-Allen, K. Janeen_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2017-08-21T14:34:16Zen_US
dc.date.available2017-08-21T14:34:16Zen_US
dc.date.issued2016en_US
dc.description.abstractAdvanced tissue engineered heart valves must be constructed from multiple materials to better mimic the heterogeneity found in the native valve. The trilayered structure of aortic valves provides the ability to open and close consistently over a full human lifetime, with each layer performing specific mechanical functions. The middle spongiosa layer consists primarily of proteoglycans and glycosaminoglycans, providing lubrication and dampening functions as the valve leaflet flexes open and closed. In this study, hyaluronan hydrogels were tuned to perform the mechanical functions of the spongiosa layer, provide a biomimetic scaffold in which valve cells were encapsulated in 3D for tissue engineering applications, and gain insight into how valve cells maintain hyaluronan homeostasis within heart valves. Expression of the HAS1 isoform of hyaluronan synthase was significantly higher in hyaluronan hydrogels compared to blank-slate poly(ethylene glycol) diacrylate (PEGDA) hydrogels. Hyaluronidase and matrix metalloproteinase enzyme activity was similar between hyaluronan and PEGDA hydrogels, even though these scaffold materials were each specifically susceptible to degradation by different enzyme types. KIAA1199 was expressed by valve cells and may play a role in the regulation of hyaluronan in heart valves. Cross-linked hyaluronan hydrogels maintained healthy phenotype of valve cells in 3D culture and were tuned to approximate the mechanical properties of the valve spongiosa layer. Therefore, hyaluronan can be used as an appropriate material for the spongiosa layer of a proposed laminate tissue engineered heart valve scaffold.en_US
dc.identifier.citationPuperi, Daniel S., O’Connell, Ronan W., Punske, Zoe E., et al.. "Hyaluronan Hydrogels for a Biomimetic Spongiosa Layer of Tissue Engineered Heart Valve Scaffolds." <i>Biomacromolecules,</i> 17, no. 5 (2016) American Chemical Society: 1766-1775. https://doi.org/10.1021/acs.biomac.6b00180.en_US
dc.identifier.digitalHyaluronan_Hydrogelsen_US
dc.identifier.doihttps://doi.org/10.1021/acs.biomac.6b00180en_US
dc.identifier.urihttps://hdl.handle.net/1911/97373en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Chemical Society.en_US
dc.titleHyaluronan Hydrogels for a Biomimetic Spongiosa Layer of Tissue Engineered Heart Valve Scaffoldsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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