Mechanical Activation of a Multimeric Adhesive Protein Through Domain Conformational Change

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dc.citation.firstpage108102en_US
dc.citation.journalTitlePhysical Review Lettersen_US
dc.citation.volumeNumber110en_US
dc.contributor.authorWijeratne, Sithara S.en_US
dc.contributor.authorBotello, Ericen_US
dc.contributor.authorYeh, Hui-Chunen_US
dc.contributor.authorZhou, Zhouen_US
dc.contributor.authorBergeron, Angela L.en_US
dc.contributor.authorFrey, Eric W.en_US
dc.contributor.authorPatel, Jay M.en_US
dc.contributor.authorNolasco, Leticiaen_US
dc.contributor.authorTurner, Nancy A.en_US
dc.contributor.authorMoake, Joel L.en_US
dc.contributor.authorDong, Jing-feien_US
dc.contributor.authorKiang, Ching-Hwaen_US
dc.contributor.orgBioengineeringen_US
dc.contributor.orgPhysics and Astronomyen_US
dc.date.accessioned2013-03-20T20:40:08Zen_US
dc.date.available2013-03-20T20:40:08Zen_US
dc.date.issued2013en_US
dc.description.abstractThe mechanical force-induced activation of the adhesive protein von Willebrand factor (VWF), which experiences high hydrodynamic forces, is essential in initiating platelet adhesion. The importance of the mechanical force-induced functional change is manifested in the multimeric VWF's crucial role in blood coagulation, when high fluid shear stress activates plasma VWF (PVWF) multimers to bind platelets. Here, we showed that a pathological level of high shear stress exposure of PVWF multimers results in domain conformational changes, and the subsequent shifts in the unfolding force allow us to use force as a marker to track the dynamic states of the multimeric VWF. We found that shear-activated PVWF multimers are more resistant to mechanical unfolding than nonsheared PVWF multimers, as indicated in the higher peak unfolding force. These results provide insight into the mechanism of shear-induced activation of PVWF multimers.en_US
dc.embargo.termsnoneen_US
dc.identifier.citationWijeratne, Sithara S., Botello, Eric, Yeh, Hui-Chun, et al.. "Mechanical Activation of a Multimeric Adhesive Protein Through Domain Conformational Change." <i>Physical Review Letters,</i> 110, (2013) American Physical Society: 108102. http://dx.doi.org/10.1103/PhysRevLett.110.108102.en_US
dc.identifier.doihttp://dx.doi.org/10.1103/PhysRevLett.110.108102en_US
dc.identifier.urihttps://hdl.handle.net/1911/70803en_US
dc.language.isoengen_US
dc.publisherAmerican Physical Societyen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.titleMechanical Activation of a Multimeric Adhesive Protein Through Domain Conformational Changeen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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