Matrix proteins are inefficiently imported into Arabidopsis peroxisomes lacking the receptor-docking peroxin PEX14

Simple item page
dc.citation.firstpage1en_US
dc.citation.issueNumber2018-01-02T00:00:00.000en_US
dc.citation.journalTitlePlant Molecular Biologyen_US
dc.citation.lastpage15en_US
dc.citation.volumeNumber77en_US
dc.contributor.authorMonroe-Augustus, Melanieen_US
dc.contributor.authorRamón, Naxhiely Martínezen_US
dc.contributor.authorRatzel, Sarah E.en_US
dc.contributor.authorLingard, Matthew J.en_US
dc.contributor.authorChristensen, Sarah E.en_US
dc.contributor.authorMurali, Chayaen_US
dc.contributor.authorBartel, Bonnieen_US
dc.date.accessioned2018-07-10T18:32:40Zen_US
dc.date.available2018-07-10T18:32:40Zen_US
dc.date.issued2011en_US
dc.description.abstractMutations in peroxisome biogenesis proteins (peroxins) can lead to developmental deficiencies in various eukaryotes. PEX14 and PEX13 are peroxins involved in docking cargo-receptor complexes at the peroxisomal membrane, thus aiding in the transport of the cargo into the peroxisomal matrix. Genetic screens have revealed numerous Arabidopsis thaliana peroxins acting in peroxisomal matrix protein import; the viable alleles isolated through these screens are generally partial loss-of-function alleles, whereas null mutations that disrupt delivery of matrix proteins to peroxisomes can confer embryonic lethality. In this study, we used forward and reverse genetics in Arabidopsis to isolate four pex14 alleles. We found that all four alleles conferred reduced PEX14 mRNA levels and displayed physiological and molecular defects suggesting reduced but not abolished peroxisomal matrix protein import. The least severe pex14 allele, pex14-3, accumulated low levels of a C-terminally truncated PEX14 product that retained partial function. Surprisingly, even the severe pex14-2 allele, which lacked detectable PEX14 mRNA and PEX14 protein, was viable, fertile, and displayed residual peroxisome matrix protein import. As pex14 plants matured, import improved. Together, our data indicate that PEX14 facilitates, but is not essential for peroxisomal matrix protein import in plants.en_US
dc.identifier.citationMonroe-Augustus, Melanie, Ramón, Naxhiely Martínez, Ratzel, Sarah E., et al.. "Matrix proteins are inefficiently imported into Arabidopsis peroxisomes lacking the receptor-docking peroxin PEX14." <i>Plant Molecular Biology,</i> 77, no. 2018-01-02T00:00:00.000 (2011) Springer: 1-15. https://doi.org/10.1007/s11103-011-9782-0.en_US
dc.identifier.digitalnihms426264en_US
dc.identifier.doihttps://doi.org/10.1007/s11103-011-9782-0en_US
dc.identifier.urihttps://hdl.handle.net/1911/102364en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Springer.en_US
dc.subject.keywordPeroxinen_US
dc.subject.keywordPeroxisome importen_US
dc.subject.keywordOrganelle targetingen_US
dc.subject.keywordArabidopsis molecular geneticsen_US
dc.titleMatrix proteins are inefficiently imported into Arabidopsis peroxisomes lacking the receptor-docking peroxin PEX14en_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
nihms426264.pdf
Size:
2.59 MB
Format:
Adobe Portable Document Format
Download
Collections
Faculty Publications
BioSciences Publications