Enhanced mutualistic symbiosis between soil phages and bacteria with elevated chromium-induced environmental stress

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dc.citation.articleNumber150en_US
dc.citation.journalTitleMicrobiomeen_US
dc.citation.volumeNumber9en_US
dc.contributor.authorHuang, Danen_US
dc.contributor.authorYu, Pingfengen_US
dc.contributor.authorYe, Maoen_US
dc.contributor.authorSchwarz, Coryen_US
dc.contributor.authorJiang, Xinen_US
dc.contributor.authorAlvarez, Pedro J.J.en_US
dc.date.accessioned2022-04-28T14:29:12Zen_US
dc.date.available2022-04-28T14:29:12Zen_US
dc.date.issued2021en_US
dc.description.abstractBackground: Microbe–virus interactions have broad implications on the composition, function, and evolution of microbiomes. Elucidating the effects of environmental stresses on these interactions is critical to identify the ecological function of viral communities and understand microbiome environmental adaptation. Heavy metal-contaminated soils represent a relevant ecosystem to study the interplay between microbes, viruses, and environmental stressors. Results: Metagenomic analysis revealed that Cr pollution adversely altered the abundance, diversity, and composition of viral and bacterial communities. Host–phage linkage based on CRISPR indicated that, in soils with high Cr contamination, the abundance of phages associated with heavy metal-tolerant hosts increased, as did the relative abundance of phages with broad host ranges (identified as host–phage linkages across genera), which would facilitate transfection and broader distribution of heavy metal resistance genes in the bacterial community. Examining variations along the pollutant gradient, enhanced mutualistic phage–bacterium interactions were observed in the face of greater environmental stresses. Specifically, the fractions of lysogens in bacterial communities (identified by integrase genes within bacterial genomes and prophage induction assay by mitomycin-C) were positively correlated with Cr contamination levels. Furthermore, viral genomic analysis demonstrated that lysogenic phages under higher Cr-induced stresses carried more auxiliary metabolic genes regulating microbial heavy metal detoxification. Conclusion: With the intensification of Cr-induced environmental stresses, the composition, replication strategy, and ecological function of the phage community all evolve alongside the bacterial community to adapt to extreme habitats. These result in a transformation of the phage–bacterium interaction from parasitism to mutualism in extreme environments and underscore the influential role of phages in bacterial adaptation to pollution-related stress and in related biogeochemical processes.en_US
dc.identifier.citationHuang, Dan, Yu, Pingfeng, Ye, Mao, et al.. "Enhanced mutualistic symbiosis between soil phages and bacteria with elevated chromium-induced environmental stress." <i>Microbiome,</i> 9, (2021) Springer Nature: https://doi.org/10.1186/s40168-021-01074-1.en_US
dc.identifier.digitals40168-021-01074-1en_US
dc.identifier.doihttps://doi.org/10.1186/s40168-021-01074-1en_US
dc.identifier.urihttps://hdl.handle.net/1911/112194en_US
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/.en_US
dc.titleEnhanced mutualistic symbiosis between soil phages and bacteria with elevated chromium-induced environmental stressen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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