Efficient synthesis of L-lactic acid from glycerol by metabolically engineered Escherichia coli
| dc.citation.journalTitle | Microbial Cell Factories | |
| dc.citation.volumeNumber | 12 | |
| dc.contributor.author | Mazumdar, Suman | |
| dc.contributor.author | Blankschien, Matthew D. | |
| dc.contributor.author | Clomburg, James M. | |
| dc.contributor.author | Gonzalez, Ramon | |
| dc.date.accessioned | 2013-03-21T14:50:33Z | |
| dc.date.available | 2013-03-21T14:50:33Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | Due to its abundance and low-price, glycerol has become an attractive carbon source for the industrial production of value-added fuels and chemicals. This work reports the engineering of E. coli for the efficient conversion of glycerol into L-lactic acid(L-lactate). Escherichia coli strains have previously been metabolically engineered for the microaerobic production of D-lactic acid from glycerol in defined media by disrupting genes that minimize the synthesis of succinate, acetate, and ethanol, and also overexpressing the respiratory route of glycerol dissimilation (GlpK/GlpD). Here, further rounds of rationale design were performed on these strains for the homofermentative production of L-lactate, not normally produced in E. coli. Specifically, L-lactate production was enabled by: 1), replacing the native D-lactate specific dehydrogenase with Streptococcus bovis L-lactate dehydrogenase (L-LDH), 2) blocking the methylglyoxal bypass pathways to avoid the synthesis of a racemic mixture of D- and L-lactate and prevent the accumulation of toxic intermediate, methylglyoxal, and 3) the native aerobic L-lactate dehydrogenase was blocked to prevent the undesired utilization of L-lactate. The engineered strain produced 50 g/L of L-lactate from 56 g/L of crude glycerol at a yield 93% of the theoretical maximum and with high optical (99.9%) and chemical (97%) purity. This study demonstrates the efficient conversion of glycerol to L-lactate, a microbial process that had not been reported in the literature prior to our work. The engineered biocatalysts produced L-lactate from crude glycerol in defined minimal salts medium at high chemical and optical purity. | |
| dc.embargo.terms | none | |
| dc.identifier.citation | Mazumdar, Suman, Blankschien, Matthew D., Clomburg, James M., et al.. "Efficient synthesis of L-lactic acid from glycerol by metabolically engineered Escherichia coli." <i>Microbial Cell Factories,</i> 12, (2013) BioMed Central: http://dx.doi.org/10.1186/1475-2859-12-7. | |
| dc.identifier.doi | http://dx.doi.org/10.1186/1475-2859-12-7 | |
| dc.identifier.uri | https://hdl.handle.net/1911/70808 | |
| dc.language.iso | eng | |
| dc.publisher | BioMed Central | |
| dc.rights | This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |
| dc.rights.uri | https://creativecommons.org/licenses/by/2.0/ | |
| dc.subject.keyword | L-lactic acid | |
| dc.subject.keyword | glycerol | |
| dc.subject.keyword | metabolic engineering | |
| dc.subject.keyword | Escherichia coli | |
| dc.title | Efficient synthesis of L-lactic acid from glycerol by metabolically engineered Escherichia coli | |
| dc.type | Journal article | |
| dc.type.dcmi | Text | |
| dc.type.publication | publisher version |