PLANT-Dx: A Molecular Diagnostic for Point-of-Use Detection of Plant Pathogens

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dc.citation.firstpage902en_US
dc.citation.issueNumber4en_US
dc.citation.journalTitleACS Synthetic Biologyen_US
dc.citation.lastpage905en_US
dc.citation.volumeNumber8en_US
dc.contributor.authorVerosloff, M.en_US
dc.contributor.authorChappell, J.en_US
dc.contributor.authorPerry, K.L.en_US
dc.contributor.authorThompson, J.R.en_US
dc.contributor.authorLucks, J.B.en_US
dc.date.accessioned2019-12-12T17:25:45Zen_US
dc.date.available2019-12-12T17:25:45Zen_US
dc.date.issued2019en_US
dc.description.abstractSynthetic biology based diagnostic technologies have improved upon gold standard diagnostic methodologies by decreasing cost, increasing accuracy, and enhancing portability. However, there has been little effort in adapting these technologies toward applications related to point-of-use monitoring of plant and crop health. Here, we take a step toward this vision by developing an approach that couples isothermal amplification of specific plant pathogen genomic sequences with customizable synthetic RNA regulators that are designed to trigger the production of a colorimetric output in cell-free gene expression reactions. We demonstrate our system can sense viral derived sequences with high sensitivity and specificity, and can be utilized to directly detect viruses from infected plant material. Furthermore, we demonstrate that the entire system can operate using only body heat and naked-eye visual analysis of outputs. We anticipate these strategies to be important components of user-friendly and deployable diagnostic systems that can be configured to detect a range of important plant pathogens.en_US
dc.identifier.citationVerosloff, M., Chappell, J., Perry, K.L., et al.. "PLANT-Dx: A Molecular Diagnostic for Point-of-Use Detection of Plant Pathogens." <i>ACS Synthetic Biology,</i> 8, no. 4 (2019) American Chemical Society: 902-905. https://doi.org/10.1021/acssynbio.8b00526.en_US
dc.identifier.digitalsb8b00526en_US
dc.identifier.doihttps://doi.org/10.1021/acssynbio.8b00526en_US
dc.identifier.urihttps://hdl.handle.net/1911/107901en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.rightsThis is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keywordpoint-of-use diagnosticsen_US
dc.subject.keywordcell free synthetic biologyen_US
dc.subject.keywordplant virusesen_US
dc.subject.keywordcrop pathogensen_US
dc.subject.keywordRNA synthetic biologyen_US
dc.subject.keywordisothermal amplificationen_US
dc.titlePLANT-Dx: A Molecular Diagnostic for Point-of-Use Detection of Plant Pathogensen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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