Metastable hybridization-based DNA information storage to allow rapid and permanent erasure

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dc.citation.articleNumber5008en_US
dc.citation.journalTitleNature Communicationsen_US
dc.citation.volumeNumber11en_US
dc.contributor.authorKim, Jangwonen_US
dc.contributor.authorBae, Jin H.en_US
dc.contributor.authorBaym, Michaelen_US
dc.contributor.authorZhang, David Yuen_US
dc.contributor.orgBioengineeringen_US
dc.contributor.orgSystems, Synthetic, and Physical Biologyen_US
dc.contributor.orgCenter for Theoretical Biological Physicsen_US
dc.date.accessioned2020-10-21T18:16:22Zen_US
dc.date.available2020-10-21T18:16:22Zen_US
dc.date.issued2020en_US
dc.description.abstractThe potential of DNA as an information storage medium is rapidly growing due to advances in DNA synthesis and sequencing. However, the chemical stability of DNA challenges the complete erasure of information encoded in DNA sequences. Here, we encode information in a DNA information solution, a mixture of true message- and false message-encoded oligonucleotides, and enables rapid and permanent erasure of information. True messages are differentiated by their hybridization to a "truth marker” oligonucleotide, and only true messages can be read; binding of the truth marker can be effectively randomized even with a brief exposure to the elevated temperature. We show 8 separate bitmap images can be stably encoded and read after storage at 25 °C for 65 days with an average of over 99% correct information recall, which extrapolates to a half-life of over 15 years at 25 °C. Heating to 95 °C for 5 minutes, however, permanently erases the message.en_US
dc.identifier.citationKim, Jangwon, Bae, Jin H., Baym, Michael, et al.. "Metastable hybridization-based DNA information storage to allow rapid and permanent erasure." <i>Nature Communications,</i> 11, (2020) Springer Nature: https://doi.org/10.1038/s41467-020-18842-6.en_US
dc.identifier.doihttps://doi.org/10.1038/s41467-020-18842-6en_US
dc.identifier.urihttps://hdl.handle.net/1911/109439en_US
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleMetastable hybridization-based DNA information storage to allow rapid and permanent erasureen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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