Differentiating between live and deadﾠMycobacterium smegmatisﾠusing autofluorescence

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dc.citation.firstpageS119en_US
dc.citation.journalTitleTuberculosisen_US
dc.citation.lastpageS123en_US
dc.citation.volumeNumber101en_US
dc.contributor.authorWong, Cynthiaen_US
dc.contributor.authorHa, Ngan P.en_US
dc.contributor.authorPawlowski, Michal E.en_US
dc.contributor.authorGraviss, Edward A.en_US
dc.contributor.authorTkaczyk, Tomasz S.en_US
dc.contributor.orgBioengineeringen_US
dc.contributor.orgElectrical and Computer Engineeringen_US
dc.date.accessioned2017-05-22T17:16:48Zen_US
dc.date.available2017-05-22T17:16:48Zen_US
dc.date.issued2016en_US
dc.description.abstractWhile there have been research efforts to find faster and more efficient diagnostic techniques for tuberculosis (TB), it is equally important to monitor a patient's response to treatment over time, especially with the increasing prevalence of multi-drug resistant (MDR) and extensively-drug resistant (XDR) TB. Between sputum smear microscopy, culture, and GeneXpert, only culture can verify viability of mycobacteria. However, it may take up to six weeks to grow Mycobacterium tuberculosis (Mtb), during which time the patient may have responded to treatment or the mycobacteria are still viable because the patient has MDR or XDR TB. In both situations, treatment incurs increased patient costs and makes them more susceptible to host-drug effects such as liver damage. Coenzyme Factor 420 (F420) is a fluorescent coenzyme found naturally in mycobacteria, with an excitation peak around 420 nm and an emission peak around 470 nm. Using Mycobacterium smegmatis, we show that live and dead mycobacteria undergo different rates of photobleaching over a period of 2 min. These preliminary experiments suggest that the different photobleaching rates could be used to help monitor a patient's response to TB treatment. In future studies, we propose to describe these experiments with Mtb as both M. smegmatis and Mtb use F420.en_US
dc.identifier.citationWong, Cynthia, Ha, Ngan P., Pawlowski, Michal E., et al.. "Differentiating between live and deadﾠMycobacterium smegmatisﾠusing autofluorescence." <i>Tuberculosis,</i> 101, (2016) Elsevier: S119-S123. https://doi.org/10.1016/j.tube.2016.09.010.en_US
dc.identifier.doihttps://doi.org/10.1016/j.tube.2016.09.010en_US
dc.identifier.urihttps://hdl.handle.net/1911/94315en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.en_US
dc.subject.keywordAutofluorescenceen_US
dc.subject.keywordMicroscopyen_US
dc.subject.keywordMycobacteriumen_US
dc.subject.keywordViabilityen_US
dc.titleDifferentiating between live and deadﾠMycobacterium smegmatisﾠusing autofluorescenceen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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