Fluorescence Correlation Spectroscopy Study of Protein Transport and Dynamic Interactions with Clustered-Charge Peptide Adsorbents

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dc.citation.firstpage435en_US
dc.citation.issueNumber8en_US
dc.citation.journalTitleJournal of Molecular Recognitionen_US
dc.citation.lastpage442en_US
dc.citation.volumeNumber25en_US
dc.contributor.authorDaniels, Charlisa R.en_US
dc.contributor.authorKisley, Lydiaen_US
dc.contributor.authorKim, Hannahen_US
dc.contributor.authorChen, Wen-Hsiangen_US
dc.contributor.authorPoongavanam, Mohan- Vivekanandanen_US
dc.contributor.authorReznik, Carmenen_US
dc.contributor.authorKourentzi, Katerinaen_US
dc.contributor.authorWillson, Richard C.en_US
dc.contributor.authorLandes, Christy F.en_US
dc.date.accessioned2013-08-22T21:49:06Zen_US
dc.date.available2013-08-22T21:49:06Zen_US
dc.date.issued2012-08en_US
dc.description.abstractIon-exchange chromatography (IEX) relies on electrostatic interactions between the adsorbent and the adsorbate, and is used extensively in protein purification. Conventional IEX utilizes ligands that are singly charged and randomly dispersed over the adsorbent, creating a heterogeneous distribution of potential adsorption sites. Clustered-charge ion exchangers exhibit higher affinity, capacity, and selectivity than their dispersed-charge counterparts of the same total charge density. In the present work, we monitored the transport behavior of an anionic protein near clusteredcharge adsorbent surfaces using Fluorescence Correlation Spectroscopy. We can resolve protein free diffusion, hindered diffusion and association with bare glass, agarose-coated, and agaroseclustered peptide surfaces, demonstrating that this method can be used to understand and ultimately optimize clustered charge adsorbent and other surface interactions at the molecular scale.en_US
dc.embargo.termsnoneen_US
dc.identifier.citationDaniels, Charlisa R., Kisley, Lydia, Kim, Hannah, et al.. "Fluorescence Correlation Spectroscopy Study of Protein Transport and Dynamic Interactions with Clustered-Charge Peptide Adsorbents." <i>Journal of Molecular Recognition,</i> 25, no. 8 (2012) Wiley-Blackwell: 435-442. http://dx.doi.org/10.1002/jmr.2206.en_US
dc.identifier.doihttp://dx.doi.org/10.1002/jmr.2206en_US
dc.identifier.urihttps://hdl.handle.net/1911/71756en_US
dc.language.isoengen_US
dc.publisherWiley-Blackwellen_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley-Blackwell.en_US
dc.subject.keywordIon-Exchange Chromatographyen_US
dc.subject.keywordProtein Adsorptionen_US
dc.subject.keyworddiffusionen_US
dc.subject.keywordAnion Exchange Matrixen_US
dc.subject.keywordClustered-Charge Ion Exchange Adsorbenten_US
dc.titleFluorescence Correlation Spectroscopy Study of Protein Transport and Dynamic Interactions with Clustered-Charge Peptide Adsorbentsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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