Fluorescence Correlation Spectroscopy Study of Protein Transport and Dynamic Interactions with Clustered-Charge Peptide Adsorbents

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dc.citation.firstpage435
dc.citation.issueNumber8
dc.citation.journalTitleJournal of Molecular Recognition
dc.citation.lastpage442
dc.citation.volumeNumber25
dc.contributor.authorDaniels, Charlisa R.
dc.contributor.authorKisley, Lydia
dc.contributor.authorKim, Hannah
dc.contributor.authorChen, Wen-Hsiang
dc.contributor.authorPoongavanam, Mohan- Vivekanandan
dc.contributor.authorReznik, Carmen
dc.contributor.authorKourentzi, Katerina
dc.contributor.authorWillson, Richard C.
dc.contributor.authorLandes, Christy F.
dc.date.accessioned2013-08-22T21:49:06Z
dc.date.available2013-08-22T21:49:06Z
dc.date.issued2012-08
dc.description.abstractIon-exchange chromatography (IEX) relies on electrostatic interactions between the adsorbent and the adsorbate, and is used extensively in protein purification. Conventional IEX utilizes ligands that are singly charged and randomly dispersed over the adsorbent, creating a heterogeneous distribution of potential adsorption sites. Clustered-charge ion exchangers exhibit higher affinity, capacity, and selectivity than their dispersed-charge counterparts of the same total charge density. In the present work, we monitored the transport behavior of an anionic protein near clusteredcharge adsorbent surfaces using Fluorescence Correlation Spectroscopy. We can resolve protein free diffusion, hindered diffusion and association with bare glass, agarose-coated, and agaroseclustered peptide surfaces, demonstrating that this method can be used to understand and ultimately optimize clustered charge adsorbent and other surface interactions at the molecular scale.
dc.embargo.termsnone
dc.identifier.citationDaniels, Charlisa R., Kisley, Lydia, Kim, Hannah, et al.. "Fluorescence Correlation Spectroscopy Study of Protein Transport and Dynamic Interactions with Clustered-Charge Peptide Adsorbents." <i>Journal of Molecular Recognition,</i> 25, no. 8 (2012) Wiley-Blackwell: 435-442. http://dx.doi.org/10.1002/jmr.2206.
dc.identifier.doihttp://dx.doi.org/10.1002/jmr.2206
dc.identifier.urihttps://hdl.handle.net/1911/71756
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley-Blackwell.
dc.subject.keywordIon-Exchange Chromatography
dc.subject.keywordProtein Adsorption
dc.subject.keyworddiffusion
dc.subject.keywordAnion Exchange Matrix
dc.subject.keywordClustered-Charge Ion Exchange Adsorbent
dc.titleFluorescence Correlation Spectroscopy Study of Protein Transport and Dynamic Interactions with Clustered-Charge Peptide Adsorbents
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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