Amniotic Fluid-Derived Stem Cells Demonstrated Cardiogenic Potential in Indirect Co-culture with Human Cardiac Cells

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dc.citation.firstpage2490en_US
dc.citation.issueNumber12en_US
dc.citation.journalTitleAnnals of Biomedical Engineeringen_US
dc.citation.lastpage2500en_US
dc.citation.volumeNumber42en_US
dc.contributor.authorGao, Yangen_US
dc.contributor.authorConnell, Jennifer Petscheen_US
dc.contributor.authorWadhwa, Lalitaen_US
dc.contributor.authorRuano, Rodrigoen_US
dc.contributor.authorJacot, Jeffrey G.en_US
dc.contributor.orgBioengineeringen_US
dc.date.accessioned2014-11-21T21:26:58Zen_US
dc.date.available2014-11-21T21:26:58Zen_US
dc.date.issued2014en_US
dc.description.abstractAmniotic fluid-derived stem cells (AFSC) have been shown to be broadly multipotent and non-tumorogenic. Previous studies of direct mixing of AFSC and neonatal rat ventricle myocytes indicated evidence of AFSC cardiogenesis. In this study, we examined human AFSC cardiogenic potential in indirect co-culture with human cardiac cells in conditions that eliminated the possibility of cell fusion. Human AFSC in contact with human cardiac cells showed expression of cardiac troponin T (cTnT) in immunohistochemistry, and no evidence of cell fusion were found through fluorescent in situ hybridization. When indirectly co-cultured with cardiac cells, human AFSC in contact with cardiac cells across a thin porous membrane showed a statistically significant increase in cTnT expression compared to non-contact conditions but lacked upregulation of calcium modulating proteins and did not have functional or morphological characteristics of mature cardiomyocytes. This suggests that contact is a necessary but not sufficient condition for AFSC cardiac differentiation in co-culture with cardiac cells.en_US
dc.identifier.citationGao, Yang, Connell, Jennifer Petsche, Wadhwa, Lalita, et al.. "Amniotic Fluid-Derived Stem Cells Demonstrated Cardiogenic Potential in Indirect Co-culture with Human Cardiac Cells." <i>Annals of Biomedical Engineering,</i> 42, no. 12 (2014) Springer: 2490-2500. http://dx.doi.org/10.1007/s10439-014-1114-5.en_US
dc.identifier.doihttp://dx.doi.org/10.1007/s10439-014-1114-5en_US
dc.identifier.urihttps://hdl.handle.net/1911/78490en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Springer.en_US
dc.subject.keywordcardiomyocytesen_US
dc.subject.keywordcardiac progenitor cellsen_US
dc.subject.keywordright ventricular out flow tracten_US
dc.subject.keywordpediatric tissue engineeringen_US
dc.subject.keywordcongenital heart defectsen_US
dc.titleAmniotic Fluid-Derived Stem Cells Demonstrated Cardiogenic Potential in Indirect Co-culture with Human Cardiac Cellsen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpost-printen_US
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