Amniotic Fluid-Derived Stem Cells Demonstrated Cardiogenic Potential in Indirect Co-culture with Human Cardiac Cells

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dc.citation.firstpage2490
dc.citation.issueNumber12
dc.citation.journalTitleAnnals of Biomedical Engineering
dc.citation.lastpage2500
dc.citation.volumeNumber42
dc.contributor.authorGao, Yang
dc.contributor.authorConnell, Jennifer Petsche
dc.contributor.authorWadhwa, Lalita
dc.contributor.authorRuano, Rodrigo
dc.contributor.authorJacot, Jeffrey G.
dc.date.accessioned2014-11-21T21:26:58Z
dc.date.available2014-11-21T21:26:58Z
dc.date.issued2014
dc.description.abstractAmniotic fluid-derived stem cells (AFSC) have been shown to be broadly multipotent and non-tumorogenic. Previous studies of direct mixing of AFSC and neonatal rat ventricle myocytes indicated evidence of AFSC cardiogenesis. In this study, we examined human AFSC cardiogenic potential in indirect co-culture with human cardiac cells in conditions that eliminated the possibility of cell fusion. Human AFSC in contact with human cardiac cells showed expression of cardiac troponin T (cTnT) in immunohistochemistry, and no evidence of cell fusion were found through fluorescent in situ hybridization. When indirectly co-cultured with cardiac cells, human AFSC in contact with cardiac cells across a thin porous membrane showed a statistically significant increase in cTnT expression compared to non-contact conditions but lacked upregulation of calcium modulating proteins and did not have functional or morphological characteristics of mature cardiomyocytes. This suggests that contact is a necessary but not sufficient condition for AFSC cardiac differentiation in co-culture with cardiac cells.
dc.identifier.citationGao, Yang, Connell, Jennifer Petsche, Wadhwa, Lalita, et al.. "Amniotic Fluid-Derived Stem Cells Demonstrated Cardiogenic Potential in Indirect Co-culture with Human Cardiac Cells." <i>Annals of Biomedical Engineering,</i> 42, no. 12 (2014) Springer: 2490-2500. http://dx.doi.org/10.1007/s10439-014-1114-5.
dc.identifier.doihttp://dx.doi.org/10.1007/s10439-014-1114-5
dc.identifier.urihttps://hdl.handle.net/1911/78490
dc.language.isoeng
dc.publisherSpringer
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Springer.
dc.subject.keywordcardiomyocytes
dc.subject.keywordcardiac progenitor cells
dc.subject.keywordright ventricular out flow tract
dc.subject.keywordpediatric tissue engineering
dc.subject.keywordcongenital heart defects
dc.titleAmniotic Fluid-Derived Stem Cells Demonstrated Cardiogenic Potential in Indirect Co-culture with Human Cardiac Cells
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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